福建农业学报2017，Vol.32Issue(10)：1051-1056,6.DOI:10.19303/j.issn.1008-0384.2017.10.001
大黄鱼肾脏组织细胞系(YCK)的建立
Development and Characterization of a New Cell Line Derived from the Kidney of Large Yellow Croaker (Larimichthys crocea)
摘要
Abstract
Large yellow croaker (Larimichthys crocea) is an important economic species in Fujian.To develop virological,toxicological and cell engineering techniques,as well as resources for advanced researches on the fish,this study had developed a new cell line derived from the fish kidneys (YCK).The obtained YCK has been successfully conserved in the M-199 medium supplemented with 10％ fetal bovine serum at 27℃ over 400 days with a medium-change every 72 h,and satisfactorily sub-cultured more than 70 times during the interim.A parallel test of commonly applied culture media was conducted to get the conclusion that M-199 being the most suitable medium for YCK.This newly established cell line consisted predominantly of epithelial-like cells,and was proven to be diploid with a modal chromosome number of 2n=48.关键词
大黄鱼/肾脏/细胞系Key words
large yellow croaker (Larimichthys crocea)/kidney/cell line分类
农业科技引用本文复制引用
郑在予,杨金先,陈秀霞,龚晖..大黄鱼肾脏组织细胞系(YCK)的建立[J].福建农业学报,2017,32(10):1051-1056,6.基金项目
福建省科技计划项目——省属公益类科研院所基本科研专项(2014R1019-8) （2014R1019-8）
福建省自然科学基金项目(2015J01106) （2015J01106）
