福建农业学报2017，Vol.32Issue(10)：1077-1081,5.DOI:10.19303/j.issn.1008-0384.2017.10.006
野莴苣及其近似种间亲缘关系研究
Genetic Comparisons Between Lactuca pulchella (Pursh) DC and Its Closely Related Lettuce Species
摘要
Abstract
The genetic similarity coefficients and cluster analysis maps on Lactuca pulchella (Lp) and its 11 closely related species were obtained by using PCR to amplify ITS,matK and rbcL gene from the total DNA fragments of the lettuce varieties.Based on the ITS sequences,a similarity between Lp and L.sativa var.ramose was found to be 93.6％;betweenLp and L.sativa,93.1％;between Lp and L.serriola,92.6％;and,between Lp and Mulgedium tataricum,the lowest at 87.8％.According to the matK sequences,a similarity between Lp and L.sativa var.ramose was shown to be99.2％;between Lp and L.sativa,99.0％,;between Lp and L.serriol,99.1％;and,between Lp and M.tataricum,98.3％.It appeared that Lpwas related closer to theLactuca species than Mulgedium.By comparing the rbcL,Lp and L.sativa L.var.ramosa had a perfect match with a 100％ similarity;whereas,Lp and L.sativa,L.indica,L.serriola,L.hirsute or L.perennis,at 99.7％;and,Lp and M.tataricum,at 99.4％.Consequently,it was concluded that Lp belonged to the genus Lactuca.关键词
野莴苣/近似种/亲缘关系Key words
Lactuca pulchella/closely related species/genetic relationship分类
生物科学引用本文复制引用
林玲,虞赟,陈智明,王德玲,沈建国,伏建国..野莴苣及其近似种间亲缘关系研究[J].福建农业学报,2017,32(10):1077-1081,5.基金项目
国家质检总局科技计划项目(2015IK043) （2015IK043）
国家质检公益性行业科研专项(201410076) （201410076）
福建省科技计划重大专项(2017NZ0003-1-7) （2017NZ0003-1-7）
