福建农业学报2017，Vol.32Issue(10)：1130-1134,5.DOI:10.19303/j.issn.1008-0384.2017.10.016
盐碱胁迫下大豆根际土壤真菌多样性分析
Fungal Diversity in Rhizosphere Soil of Soybean Fields under Salt-alkali Stress
摘要
Abstract
To understand the adaptability of fungi in rhizosphere soil of soybean,two saline-alkali stress ways were set which involved irrigating saline solution (A) and covering saline soil (B),to analyze the diversity of rhizospherical fungi of wild soybean and cultivated soybean,throughthe application of DGGE method.The results showed that the saline alkali stress ways had great influence on the similarity of fungi in rhizosphere of soybean.For example,under the same saline alkali stress way and concentration of A1,the similarity ratio between the rhizospherical fungi of wild soybean and cultivated soybean was 0.70.In addition,appropriate saline concentration can increase the uniformity and Shannon index of soybean rhizospherical fungi,for example,the uniformity of wild soybean at concentration of B1 was 0.99,and the Shannon index at concentration of A2 was 1.87.Moreover,the sequencing of DGGE specific bands and the further comparison with gene bank,some comparative advantageous strain were obtained,such as Fusarium oxysporum,Actinomucor elegans,Marcelleina tuberculispora,Marcelleina persoonii,To fieldiaceae environmental sample clone and Uncultured eukaryote clone etc.关键词
野生大豆/栽培大豆/根际真菌/DGGEKey words
wild soybean (Glycine soja)/cultivated soybean (Glycine max)/rhizospherical fungi/DGGE分类
生物科学引用本文复制引用
张建峰,吉丽,蔺朝龙,田磊,孔钰凤,田春杰..盐碱胁迫下大豆根际土壤真菌多样性分析[J].福建农业学报,2017,32(10):1130-1134,5.基金项目
吉林省教育厅项目(2016176) （2016176）
国家重点研发计划项目(2016YFC0501202) （2016YFC0501202）
吉林省科技厅项目(20160412017XH) （20160412017XH）
