法医学杂志2018,Vol.34Issue(1):33-36,4.DOI:10.3969/j.issn.1004-5619.2018.01.007
示指与接触物菌群ERIC-PCR指纹图谱比对
Comparison of Bacteria ERIC-PCR Fingerprints of Index Fingers and Contactants
摘要
Abstract
Objective To explore the bacteria relevance between index fingers and contactant' surfaces (mobile phone touch screen and desktop of personal office table). Methods Bacteria were collected from the index fingers, mobile phone touch screen and desktop of personal office table of 10 volunteers. Enterobacterial repetitive intergenic consensus(ERIC)-PCR fingerprint was established by PCR amplifi-cation technique of metagenome. Results There were 7 volunteers' ERIC-PCR fingerprints of index fin-gers matched that took from the mobile phone touch screens, and different from each other. There were 3 volunteers' ERIC-PCR fingerprints of index fingers matched that took from desk top of personal office table, and other 7 volunteers' ERIC-PCR fingerprints did not match perfectly with that took from desk top of personal office table,but had at least one similar band for both. Conclusion The bacteria on index finger shows individual specificity, which on mobile phone touching screen and personal desktop may be a new biological sample of forensic identification.关键词
法医遗传学/聚合酶链反应/DNA指纹法/肠道细菌基因间重复序列/指纹图谱Key words
forensic genetics/polymerase chain reaction/DNA fingerprinting/enterobacterial repetitive in-tergenic consensus/finger print分类
社会科学引用本文复制引用
刘耘汀,孙大明,施少培,杨旭..示指与接触物菌群ERIC-PCR指纹图谱比对[J].法医学杂志,2018,34(1):33-36,4.基金项目
国家社会科学基金资助项目(17BFX063) (17BFX063)
上海市法医学重点实验室资助项目(17DZ2273200) (17DZ2273200)
上海市司法鉴定专业技术服务平台资助项目(16DZ2290900) (16DZ2290900)