国际病理科学与临床杂志2017，Vol.37Issue(11)：2291-2298,8.DOI:10.3978/j.issn.2095-6959.2017.11.002
异槲皮苷对成骨细胞分化及HDAC1和Runx2表达的影响
Effect of isoquercetin on osteoblast differentiation and expression level of HDAC1 and Runx2
摘要
Abstract
Objective:To explore effect of the isoquercetin on osteoblast differentiation and expression level of HDAC 1 and Runx2.Methods:The type 1 collagen (Co11),alkaline phosphatase (ALP) and osteopontin (OPN) expression level in MC3T3-E1 cells was detected by using qRT-PCR after treated with 1×10 8,1×10-7,1×10-6,1×10-5 mol/L isoquercetin.T-he ALP activity was assayed using ALP detection kit.Mineralization of MC3T3-E1 was evaluated by Alizarin red staining.What's more,the mRNA levels of HDAC1 and Runx2 in MC3T3-E1 cells were observed by using qRT-P CR post-treatment by 1 × 10-8,1 × 10-7,1 × 10-6,1 × 10-5 mol/L isoquercetin.Results:T-he Col 1,ALP and OPN expression level turned significantly higher after treated with 1 × 10-8,1 × 10-7,1 × 10-6,1 × 10-5 mol/L isoquercetin (P＜0.05).There was a certain dose effect between them.As the same,the ALP activity and mineralization of MC3T3-E 1 was higher post-exposure to the isoquercetin.HDAC 1 in the MC3T3-E 1 cells had lower expression level after treated with the isoquercetin (P＜0.05).However,Runx2 in the MC3T3-E1 cells had higher expression level after exposed to the isoquercetin (P＜0.05).Conclusion:The isoquercetin can promote the expression of Runx2 through inhibiting the expression of HDAC 1,in order to improve the differentiation of osteoblast.The results provide a new theoretical basis for the application ofisoquercetin in the treatment of osteoporosis.关键词
异槲皮苷/成骨细胞分化/Ⅰ型胶原蛋白/碱性磷酸酶/HDAC1/Runx2Key words
isoquercetin/osteoblast differentiation/type 1 collagen/alkaline phosphatase/HDAC1/Runx2引用本文复制引用
季泽娟,张春旭,郭占豪,刘方娜,刘鑫..异槲皮苷对成骨细胞分化及HDAC1和Runx2表达的影响[J].国际病理科学与临床杂志,2017,37(11):2291-2298,8.基金项目
河南省医学科技攻关计划项目(201503048).This work was supported by Henan Province Medical Science and Technology Research Project,China (201503048). （201503048）
