临床与病理杂志2017，Vol.37Issue(11)：2299-2304,6.DOI:10.3978/j.issn.2095-6959.2017.11.003
Ku80沉默增强鼻咽癌细胞放射敏感性并诱导凋亡
Silencing expression of Ku80 enhances the radiosensitivity and induces apoptosis in nasopharyngeal carcinoma cell
柳艳飞 1金红艳 1田勇 1王晓凤 1何为 1韩云峰2
作者信息
- 1. 武汉市普仁医院肿瘤科,武汉430081
- 2. 华中科技大学同济医学院附属同济医院核医学科,武汉430030
- 折叠
摘要
Abstract
Objective:To investigate the effect of silencing Ku80 expression on radiosensitivity and apoptosis in nasopharyngeal carcinoma cell.Methods:qRT-PCR was used to measure the expression level of Ku80 mRNA in nasopharyngeal carcinoma cell and normal nasopharyngeal cell.The CNE2 cells were divided into three groups:a control group transfect with no lentiviral,a negative control ShRNA group transfect with negative control lentiviral,and a Ku80 ShRNA group transfect with Ku80 shRNA lentiviral.The transfect efficiency was compared between the 3 groups.These 3 groups were irradiated with 0,2,4,6,8 and 10 Gy.The cell survival rate was measured and compared in these 3 groups.And apoptosis rate was also tested.Results:The expression levels of Ku80 mRNA in CNE2,CNE1,HONE-1 and C666 cell line were 9.0±0.9,8.0±0.3,5.0±0.4,4.0±0.2,which was significantly higher than 1.0 in the normal nasopharyngeal epithelium cell line,NP69,the difference was statistically significant (P＜0.05).The expression level of Ku80 mRNA in Ku80 shRNA group was 0.1±0.03,which was significantly lower than 0.93±0.04 in the negative control ShRNA group,the difference was statistically significant (P＜0.001).The expression level of Ku80 protein was 0.15±0.05 in the Ku80 shRNA group after lentiviral transfection,which was significantly lower than 1.0 in the negative control ShRNA group (P＜0.001).The cell survival rate in the Ku80 shRNA group was significantly lower than that in the control and the negative shRNA control group.The apoptosis rate of the Ku80 ShRNA group was 20.0％±0.9％,which was significantly higher than 4.3％ ±0.8％ in the control group and 5.0％±0.8％ in the negative shRNA control group.Conclusion:Silencing of Ku80 via shRNA interference can enhance the radiosensitivity and induce apoptosis in nasopharyngeal carcinoma cell.Silencing Ku80 expression may become a target of enhancement of the radiosensitivity in nasopharyngeal carcinoma.关键词
鼻咽癌/放射敏感性/Ku80/凋亡Key words
nasopharyngeal carcinoma/radiosensitivity/Ku80/apoptosis引用本文复制引用
柳艳飞,金红艳,田勇,王晓凤,何为,韩云峰..Ku80沉默增强鼻咽癌细胞放射敏感性并诱导凋亡[J].临床与病理杂志,2017,37(11):2299-2304,6.
