临床与病理杂志2017，Vol.37Issue(11)：2305-2309,5.DOI:10.3978/j.issn.2095-6959.2017.11.004
达卡巴嗪诱导黑素瘤耐药细胞系的建立及其耐药性
Establishment of a dacarbazine-induced resistance melanoma cell line and its drug resistance
摘要
Abstract
Objective:To establish a melanoma cell line resistant to dacarbazine (DTIC) and to investigate its drug resistance.Methods:O6-methylguananine-DNA methyltransferase (MGMT) protein expression of a human melanoma cell line was detected using Western blot.The drug resistance of human melanoma A375 cells to DTIC was induced by in vitro stepwise induction.Drug-resistant cell line IC50 and resistance index were detected by MTI assay.Results:After treated with DTIC at different concentrations,the growth ofA375 cells slowed down in different degrees.With the increase of DTIC concentration,the inhibition rate ofA375 cells increased in different degrees.The median inhibitory concentration (IC50) of DTIC to in vitro proliferation of parent A375 cells and A375/DTIC cells was 10.766 and 95.693,respectively.The resistance index of A375/DTIC cells to DTIC was 9 times higher than that ofA375 cells (P＜0.05).Conclusion:The successful establishment of a DTIC-induced drug-resistant human melanoma cell line A375/DTIC in vitro by gradually increasing the concentration of chemotherapeutic drugs with stages lays an experimental foundation for further study on the mechanism of melanoma resistant to DTIC.关键词
黑素瘤/达卡巴嗪/O6-甲基鸟嘌呤-DNA甲基转移酶Key words
melanoma/dacarbazine/O6-methylguananine-DNA methyltransferase引用本文复制引用
孙金中,孙圣荣,蒋冠..达卡巴嗪诱导黑素瘤耐药细胞系的建立及其耐药性[J].临床与病理杂志,2017,37(11):2305-2309,5.基金项目
湖北省自然科学基金(2017CFB628).This work was supported by Hubei Provincial Natural Science Foundation,China(2017CFB628). （2017CFB628）
