临床与病理杂志2017，Vol.37Issue(11)：2339-2344,6.DOI:10.3978/j.issn.2095-6959.2017.11.009
神经母细胞源性肿瘤患儿N-MYC基因拷贝数的变化及其临床意义
Copy number variations and its clinical significance of N-MYC gene in children with neuroblastic tumors
王沛 1关丹丹 1岳娉 1许素素 1宫丽平 1袁远2
作者信息
- 1. 首都医科大学基础医学院病理系,北京100069
- 2. 肿瘤侵袭和转移机制研究北京市重点实验室,北京100069
- 折叠
摘要
Abstract
Objective:To detect N-MYC gene copy number alterations,and to analyze their related clinicopathological implications in pediatric neuroblastic tumors (NTs).Methods:A total of 483 NT samples were obtained,including 388 neuroblastomas (NBs),89 ganglioneuroblastomas (GNBs) and 6 ganglioneuromas (GNs).Fluorescence in situ hybridization (FISH) was used to detect numerical aberrations of N-MYC.Statistical analysis was used to study its association with clinicopathological features,as well as with the survival rate of patients.Results:Of 483 NT cases,N-MYC amplification rate was 12.4％.Gene amplification of N-MYC were found only in NB,but not in any GNB or GN case (P＜0.05).N-MYC gene amplification was more likely to occur in poorly differentiated neuroblastoma (P=0.01),the rate of which was inversely related to tumor differentiation.The N-MYC amplification rate in male patients was higher than that in female patients (P=0.05).There was a trend that the amplification rate of N-MYC gene was lower in patients ≤18 months as compared to patients ＞18 months,but difference was not significantly (P=0.092).The univariate survival analysis showed that the survival rate of patients with N-MYC gene amplification was significantly lower than those with N-MYC gain or normal gene status.Conclusion:N-MYC gene amplification is tightly correlated with tumor type,differentiation,gender,age and surwval.Detection of abnormal N-MYC copy number would be helpful for the diagnosis and prognosis ofneuroblastic tumors.关键词
神经母细胞源性肿瘤/N-MYC/荧光原位杂交技术/临床病理分析Key words
neuroblastic tumors/N-MYC gene/fluorescence in situ hybridization/clinicopathological analysis引用本文复制引用
王沛,关丹丹,岳娉,许素素,宫丽平,袁远..神经母细胞源性肿瘤患儿N-MYC基因拷贝数的变化及其临床意义[J].临床与病理杂志,2017,37(11):2339-2344,6.
