国际病理科学与临床杂志2017，Vol.37Issue(12)：2517-2523,7.DOI:10.3978/j.issn.2095-6959.2017.12.001
HCN1,HCN2在功能性便秘大鼠结肠的分布及其与Cajal间质细胞的共存关系
Distribution of HCN1, HCN2 in colon of functional constipation rats and its co-localization with interstitial cells of Cajal
摘要
Abstract
Objective:To investigate the distribution of HCN1,HCN2 in colon of functional constipation rats and its colocalization with interstitial cells of Cajal (ICCs) for study on HCN1,HCN2 which play the role of the onset of action in functional constipation.Methods:Twenty-four SD female rats in 12-week were randomized into a normal group (n=12) and a model group (n=12).The immunofluorescence double-labeling stain was used to detect the distribution of HCN1,HCN2,ICC in mucosa of ascending colon,transverse colon and descending colon in functional constipation rats triggered by compound diphenoxylate.Results:HCN1,HCN2 and ICC expressed in mucosa of ascending colon,transverse colon and descending colon tend to be positive after double libeling with c-Kit both in normal and model group.HCN1 expression mainly distributed in ICC-MY between Ring muscle and Longitudinal muscle which was the same with c-Kit expression.HCN2 positive neurons was close to ICCs,which had no co-localization.Conclusion:HCN channel which is closely related with independent blogging activity may participate in colon ICCs independent blogging activity.HCN1,HCN2 may take part in regulation of colon motility.The number,function and the distribution may be related to pathogenesis of functional constipation.关键词
功能性便秘/HCN1/HCN2/结肠Cajal间质细胞Key words
functional constipation/HCN1/HCN2/the colon Cajal mesenchymal cells引用本文复制引用
肖秋平,贾玉龙,陈云艳,耿学斯..HCN1,HCN2在功能性便秘大鼠结肠的分布及其与Cajal间质细胞的共存关系[J].国际病理科学与临床杂志,2017,37(12):2517-2523,7.基金项目
2012年厦门市科技局指导性项目(201209) （201209）
2016年福建省卫生和计划生育委员会青年科研项目(2016-2-73).This work was supported by the Plan from Xiamen Science and Technology Program (201209) and the Project from Youth Foundation of Health Department of Fujian Province (2016-2-73),China. （2016-2-73）
