临床与病理杂志2017，Vol.37Issue(12)：2536-2542,7.DOI:10.3978/j.issn.2095-6959.2017.12.004
YAP对卵巢癌干细胞增殖的影响
Effect of YAP gene on proliferation of ovarian cancer stem cells
许静 1郭哲 1王秋宇 1梁殿迅1
作者信息
- 1. 南阳市中心医院妇产科,河南南阳473000
- 折叠
摘要
Abstract
Objective:To investigate the role of YAP in ovarian cancer stem cells and its influence on biologic function.Methods:A single cell suspension was prepared after digestion of pancreatic enzymes with OVCAR-3 cells.And then,the ovarian cancer stem cells were identified by flow cytometry.The YAP five phosphorylation site mutation (YAP-5SA) and C terminal deletion (YAP-△ C) in the construction of vector lentiviral packaging vector,and the virus core particles transfected with YAP-5SA,YAP-△ C vector in ovarian cancer stem cells,then the cell proliferation and stability were examined by MTT assay and colonyforming assay.The ovarian cancer stem cells,YAP-5SA and YAP-△ C transfected ovarian cancer stem cells were inoculated in 15 the nude mice,the volume of tumors were measurand for 4 weeks,once a week.Results:After the ovarian cancer stem cells inoculated in DMEM culture medium for 24 h,the number of cells were increased,and 5 days the cells presenting stem cell globules and covering the bottom of the bottle.And the cells had 99.8％ CD44 and 0.76％ CD117 examined by flow cytometry.YAP level was higher in ovarian cancer stem cells than ovarian cancer cells.And YAP-5SA promoted stem cell proliferation and tablet cell cloning in ovarian cancer stem cells,however,YAP-△ C showed the opposite phenomenon.YAP-5SA transfected ovarian cancer stem cells promoted the growth of the tumor,however,YAP-△ C transfected ovarian cancer stem cells suppressed the growth of the tumor.Condusion:These results suggest that YAP contribute to the proliferation of ovarian stem cells and participate in the development and progression of ovarian cancer.关键词
卵巢干细胞/增殖/克隆形成/YAP信号/人卵巢细胞株OVCAR-3Key words
ovarian cancer stem cells/proliferation/colony-forming/YAP signaling/human ovarian cell line OVCAR-3引用本文复制引用
许静,郭哲,王秋宇,梁殿迅..YAP对卵巢癌干细胞增殖的影响[J].临床与病理杂志,2017,37(12):2536-2542,7.
