临床与病理杂志2017，Vol.37Issue(12)：2555-2560,6.DOI:10.3978/j.issn.2095-6959.2017.12.007
血清ACE2在评估胆道闭锁肝纤维化及其预后中的应用价值
Value of serum ACE2 in assessing liver fibrosis and prognosis for biliary atresia
吴一波 1吕志宝 1徐伟珏 1刘江斌 1郑露露1
作者信息
- 1. 上海交通大学附属儿童医院普外科,上海200062
- 折叠
摘要
Abstract
Objective:To understand the influence factors for liver fibrosis,to explore the expression and serum concentration of angiotensin converting enzyme 2 (ACE2) and liver fibrosis in biliary atresia (BA),and to determine the relationship between serum ACE2 and prognosis in children suffering from postoperative BA.Methods:A total of 35 BA infants (the BA group) and 14 choledochocyst ones (the control group) who donated the blood and liver tissue were enrolled in this study to figure out the ACE2 by real time polymerase chain reaction and ELISA methods.The extent of fibrosis in liver samples was blindly evaluated by two experienced pathologists depending on Ishak system.Then the BA liver samples were divided into a mild liver fibrosis group (grade Ⅰ to Ⅳ,Fa) and a severe liver fibrosis group (grade Ⅴ to Ⅵ,Fb).Results:1) Compared to the control group,ACE2 gene (0.42±0.3 vs 1.0±0.2,P=0.03) and ACE2 protein [(3.4±0.8) μg/μL vs (4.8±0.5) μg/μL,P=0.04] decreased significantly in BA group.2) Compared to severe liver fibrosis group,ACE2 relative protein increased significantly [(3.8±0.94) μg/μL vs (2.5±0.39) μg/μL,P＜0.01].3) In mild liver fibrosis,ACE2 relative protein increased significantly when compared to pre-operative [(5.09±0.96) μg/μL vs (3.80±0.94) μg/μL,P＜0.01].In severe liver fibrosis group,there were no difference between post-and pre-operative [(2.57±1) μg/μL vs (2.52±0.39) μg/μL,P=0.87].ACE2 protein in the Jaun group was significantly higher than that in the No-Jaun group (5.3±0.7 vs 2.9±0.9,P=0.003).Conclusion:ACE2 decreases in BA group,and is associated with liver fibrosis,which may be used for evaluation of prognosis.关键词
胆道闭锁/血管紧张素转化酶2/肝纤维化Key words
biliary atresia/angiotensin-converting enzyme 2/liver fibrosis引用本文复制引用
吴一波,吕志宝,徐伟珏,刘江斌,郑露露..血清ACE2在评估胆道闭锁肝纤维化及其预后中的应用价值[J].临床与病理杂志,2017,37(12):2555-2560,6.
