临床与病理杂志2017，Vol.37Issue(12)：2583-2588,6.DOI:10.3978/j.issn.2095-6959.2017.12.012
支气管哮喘患儿外周血中ADAM8,IRF1的表达水平及其临床意义
ADAM8 and IRF1 expression levels in peripheral blood of children with bronchial asthma and clinical significance
摘要
Abstract
Objective:To explore disintegrins metalloproteinase 8 (ADAM8) and interferon regulatory factor 1 (IRF1) expression levels in peripheral blood of children with bronchial asthma and clinical significance.Methods:A total of 83 cases of children with bronchial asthma treated in our hospital from March 2015 to March 2017 were selected as the study objects and divided into a mild persistent group (n=47),a moderate group (n=19),and a severe group (n=17) according to Global Asthma Initiative (GINA);35 healthy children in the same period were as the control group.ADAM8 and IRFI mRNA levels in peripheral blood of children in each group were detected by fluorescent quantitative PCR (qRT-PCR),ADAM8 and IRF1 protein levels in peripheral blood of children in each group were tested by Western blot,lung function in each group was tested by lung function tester.The relevance of ADAM8,IRF1 protein levels and lung function was analyzed.Results:Compared with those in the control group,ADAM8,IRF1 mRNA and protein levels in peripheral blood of asthmatic children significantly increased (P＜0.05).ADAM8,IRF1 mRNA and protein levels in peripheral blood of children with moderate and severe asthma were significantly higher than those of mild children (P＜0.05).ADAM8,IRF1 mRNA and protein levels in peripheral blood of asthmatic children were negatively correlated with the forced expiratory volume in 1 second (FEV1),forced expiratory volume in 1 second and forced vital capacity ratio (FEV1/FVC),forced peak expiratory flow (PEF) and other lung function indexes.Conclusion:ADAM8 and IRF1 high expression in peripheral blood of asthmatic children is negatively related to lung function,with synergistic proinflammatory effect.It is suggested that ADAM8 and IRF1 can be used as reference indexes for evaluating the development of asthma in clinic,and the two can be used as a new target for asthma treatment.关键词
支气管哮喘/去整合素金属蛋白酶8/干扰素调控因子1/儿童/肺功能Key words
bronchial asthma/integrin metalloproteinase 8/interferon regulatory factor 1/children/pulmonary function引用本文复制引用
梁雯慧,庄帝钱,刘雅玲,郭瑞雪..支气管哮喘患儿外周血中ADAM8,IRF1的表达水平及其临床意义[J].临床与病理杂志,2017,37(12):2583-2588,6.基金项目
深圳市科技计划项目(JCY20150403150555531). This work was supported by Shenzhen Science and Technology Plan Project,China (JCY20150403150555531). （JCY20150403150555531）
