临床与病理杂志2017，Vol.37Issue(12)：2601-2604,4.DOI:10.3978/j.issn.2095-6959.2017.12.015
IL-22基因启动子区甲基化检测在宫颈癌中的临床意义
Clinical significance of promoter region methylation of IL-22 gene in uterine cervical cancer
肖义森 1潘国基 1杨丽梅 1李雪梅2
作者信息
- 1. 惠州仲恺高新区人民医院检验科,广东惠州516229
- 2. 惠州仲恺高新区人民医院妇产科,广东惠州516229
- 折叠
摘要
Abstract
Objective:To detect promoter region methylation of IL-22 gene in uterine cervical cancer (UCC) and explore its clinical significance.Methods:One hundred and four uterine cervical cancer patients from Huizhou Zhongkai High-tech Zone of People's Hospital were enrolled in this study.Another 60 patients who underwent total hysterectomy for benign diseases was used as control,during the same period.Methylation of the IL-22 promoter was detected using specific PCR (MSP).IL-22 expression was detected by ELISA.Results:The positive rate of IL-22 promoter methylation and IL-22 expression in UCC patients was 32.7％ (34/104) and (28.36±5.48) ng/L,which was significantly higher than that of 15.0％(9/60) and (14.52±1.37) ng/L in control group (P＜0.05).The positive rate ofIL-22 methylation and IL-22 expression in Ⅲ and Ⅳ stage group was 40.4％ (19/47) and (30.71±6.11) ng/L,which was both higher than that of 26.3％ (15/57) and (27.42±4.74) ng/L in Ⅰ and Ⅱ stage group (P＜0.05).The positive rate of IL-22 methylation and IL-22 expression in lymph node metastasis group was 42.1％ (16/38) and (31.02±6.58) ng/L,which was both higher than that of 27.3％ (18/66) and (27.42±4.74) ng/L in non-lymph node metastasis group (P＜0.05).The positive rate of IL-22 methylation and IL-22 expression in median and low differentiation group was 36.6％ (26/71) and (30.13±6.37) ng/L,which was both higher than that of 24.2％ (8/33) and (24.56±4.85) ng/L in well differentiation group (P＜0.05).Conclusion:High-methylation of IL-22 was detected in UCC,which increased IL-22 expression.The expression changes may be involved in the occurrence,development and metastasis of cervical cancer.关键词
宫颈癌/IL-22/甲基化修饰Key words
uterine cervical cancer/IL-22/methylation引用本文复制引用
肖义森,潘国基,杨丽梅,李雪梅..IL-22基因启动子区甲基化检测在宫颈癌中的临床意义[J].临床与病理杂志,2017,37(12):2601-2604,4.
