临床与病理杂志2017，Vol.37Issue(12)：2625-2632,8.DOI:10.3978/j.issn.2095-6959.2017.12.019
通血健骨丸对兔缺血性坏死股骨头HIF-1α,VEGF和BMP-2表达的影响
Effect of blood invigorating bone pill on HIF-1α, VEGF and BMP-2 expression of rabbits with avascular necrosis of femoral head
摘要
Abstract
Objective:To explore the influence of blood invigorating bone pill on hypoxia inducible factor 1 α (HIF-1α),vascular endothelial growth factor (VEGF) and bone morphogenetic protein (BMP-2) expression of rabbits with avascular necrosis of femoral head.Methods:The model of rabbit with avascular necrosis of femoral head was induced with prednisolone acetate by gluteal injection,and the normal control group were not given any treatment.After modeling,general physiological condition of normal control group and model group were observed,and the morphology of femoral head was examined by using naked eye and X-ray.The model were divided into four groups,and named for model group,blood invigorating bone pill low dose group,blood invigorating bone pill medium-dose group,and blood invigorating bone pill high dose group,with 300,400,500 mg/kg,respectively.The normal control group and model group were given the same volume of normal saline,and gastric lavage treatment.HIF-1α,VEGF and BMP-2 expression was detected by using real time quantitative PCR (qRT-PCR) and Western blot.Results:After modeling,there was less appetite,less mental activity,and limited range of activities in the model group.In the model group,the surface of femoral head was depressed,the bone density was uneven,the weight-bearing area was cystic,the joint space was narrow,the subchondral fat cells increased,the trabecular bone became thin and the bone cells atrophied.HIF-1α,VEGF and BMP-2 expression in the model group were significantly lower than those in the normal control group (P＜0.05).HIF-1α,VEGF and BMP-2 expression in the blood strengthening bone pill group were significantly higher than those in the model group (P＜0.05),HIF-1α,VEGF and BMP-2 increased significantly with with the increase in the amount of blood strengthening bone pill (P＜0.05),showing dose-dependent.Conclusion:Blood invigorating bone pill can promote HIF-1α,VEGF and BMP-2 up-regulation expression of rabbits with avascular necrosis of the femoral head,and then promote bone repair.关键词
通血健骨丸/缺血性股骨头坏死/低氧诱导因子1α/血管内皮生长因子/骨形态发生蛋白2Key words
blood invigorating bone pill/avascular necrosis of femoral head/hypoxia inducible factor 1α/vascular endothelial growth factor/bone morphogenetic protein 2引用本文复制引用
王江伟,魏道德,王丽,李松伟..通血健骨丸对兔缺血性坏死股骨头HIF-1α,VEGF和BMP-2表达的影响[J].临床与病理杂志,2017,37(12):2625-2632,8.基金项目
河南省医学科技攻关计划项目(201504152).This work was supported by the Henan Medical Science and Technology Research Project,China(201504152). （201504152）
