广西医科大学学报2018，Vol.35Issue(2)：146-148,3.DOI:10.16190/j.cnki.45-1211/r.2018.02.002
维生素B3促进大鼠中性粒细胞生成的机制研究
The effect and mechanism of Vitamin B3 on granulopoiesis in rats
摘要
Abstract
Objective:To investigate the effect and mechanism of Vitamin B3 (Vit B3)on granulopoiesis in normal rats.Methods:21 healthy female SD rats were randomly divided into three groups (n=7 per group):the control group in which the rats were given 9％ NaCl via gavage,the rhG-CSF group in which the rats were given 10 μg/d rhG-CSF via subcutaneous injection for 7 days,and the Vit B3 group in which the rats were given 500 mg/kg rhG-CSF for 7 days.The neutrophil granulocyte counts were analyzed before treatment and the 7th day after administration.The serum cytokines such as granulocyte macrophage colony stimulating factor (GM-CSF),tumor necrosis factor (TNF)-α,interleukin (IL)-1β,monocyte chemoattractant protein-1 (MCP-1),macrophage inflammatory protein-lα (MIP-lα)and tumor growth related factors GRO/KC levels were detected by Bio-Plex suspension array system.Results:The neutrophil counts increased significantly in Vit B3 and rhG-CSF groups (P＜0.05),but there was no significant difference between the rhG-CSF group and the Vit B3 group (P＞0.05).The levels of TNF-α,IL-1β,MCP-1 and GRO/KC in serum were significantly increased by Vit B3 administration (P＜0.05).The serum TNF-α level was increased by G-CSF treatment (P＜0.05).The levels of IL-1β,MCP-1 and GRO/KC were markedly higher in the Vit B3 group than those in the rhG-CSF group (P＜0.05).Conclusion:Vit B3 could result in increasing absolute neutrophil count in healthy rats,and the mechanism might be related to the elevation of serum inflammatory factors TNF-α,IL-1β,MCP-1 and GRO/KC levels.关键词
Bio-Plex悬液芯片系统/维生素B3/中性粒细胞生成/细胞因子/rhG-CSFKey words
Bio-Plex suspension array system/Vitamin B3/granulopoiesis/cytokine/rhG-CSF分类
医药卫生引用本文复制引用
杨莹,王梦婕,杨湖,李新叶,蓝丹..维生素B3促进大鼠中性粒细胞生成的机制研究[J].广西医科大学学报,2018,35(2):146-148,3.基金项目
国家自然科学基金资助项目(No.81060045) （No.81060045）
