广西医科大学学报2018，Vol.35Issue(2)：162-165,4.DOI:10.16190/j.cnki.45-1211/r.2018.02.006
龙琥醒脑颗粒对脑出血模型小鼠cAMP/PKA/AQP4信号通路的调控作用
Effect of Longhu Xingnao granule on intracerebral hemorrhage in rats and the regulation of cAMP/PKA/AQP4 signaling pathway
摘要
Abstract
Objective:To investigate the effect of Longhu Xingnao granule (LXG) on intracerebral hemorrhage in rats and the regulation of cAMP/PKA/AQP4 signaling pathway.Methods:A mouse ICH model was established by Rynkowski method.Sixty male ICH mice were randomly divided into normal group,sham operation group,model group,LXG high-dose (20 g/kg) group and low-dose (10 g/kg) group.Mice in high-dose and low-dose groups were given LXG via gavage for two weeks after modeling,and mice in other groups were given equal volume of saline.Neurological deficits was assessed by Longa'method on day 7 and day 14 after administration.Brain edema was quantitated by dry/wet weight.The mRNA and protein expressions of cAMP,PKA and AQP4 in brain tissues were detected by qPCR and western blotting,respectively.Results:Neurological deficits grade and brain water content were markedly increased in the model group when compared with the normal group and sham operation group (P＜0.05).Compared with model group,neurological deficits grade and brain tissue water content were obviously decreased in LXG high-dose group and low-dose group (P＜0.05).The mRNA and protein expressions of cAMP,PKA and AQP4 in brain tissues were notably down-regulated in LXG high-dose group and low-dose group (P＜0.05).Conclusion:LXG attenuated neurological impairment and encephaledema in ICH mice probably via the regulation of the cAMP/PKA/AQP4 signaling pathway.关键词
脑出血/龙琥醒脑颗粒/cAMP/PKA/AQP4/动物模型/小鼠Key words
intracerebral hemorrhage/Longhu Xingnao granule/cAMP/PKA/AQP4/animal model/mice分类
医药卫生引用本文复制引用
余文运,喻坚柏,罗刚..龙琥醒脑颗粒对脑出血模型小鼠cAMP/PKA/AQP4信号通路的调控作用[J].广西医科大学学报,2018,35(2):162-165,4.基金项目
长沙市科技计划项目(No.KQ1606006) （No.KQ1606006）
