广西医科大学学报2018，Vol.35Issue(2)：166-169,4.DOI:10.16190/j.cnki.45-1211/r.2018.02.007
血府逐瘀汤对重型颅脑损伤大鼠脑细胞凋亡及相关蛋白Bcl-2、Bax的影响
Effects of Xuefu Zhuyu decoction on brain cell apoptosis and Bcl-2/Bax expression in rats with severe traumatic brain injury
摘要
Abstract
Objective:To investigate the effect and mechanisms of Xuefu Zhuyu decoction on severe traumatic brain injury (STBI) in rats.Methods:A total of 72 SD rats were randomly divided into group A (normal control group),group B (model group) and group C (Xuefu Zhuyu decoction group),with 24 cases in each group.Modified Feeney's method was used to establish a rat model of STBI.After 6 h of modeling,the rats in group C were given Xuefu Zhuyu decoction by gavage,and rats in group A and B were given equal volume of saline.Eight rats from each group were sacrificed at 24 h,72 h and 168 h post-modeling.The neurological deficit score (NSS) and the apoptosis rate of brain cells were detected.The protein expressions of Bcl-2 and Bax were determined by immunohistochemistry.Results:Compared with group A,the NSS score in group B was significantly increased (P＜0.05),and reached peak at 24 h,then gradually decreased and came to its lowest level at 168 h.However,the NSS score in group C was markedly decreased compared with group B (P＜0.05).The apoptosis rate of brain cells in group B was higher than that in group A,as evidenced by TUNEL staining (P＜0.05).It peaked at 72 h and then decreased.Compared with group B,the apoptosis rate of brain cells in group C was reduced significantly (P＜0.05).There was no obvious change in the apoptosis rate from 24 h to 72 h in group C (P＞0.05),but it reduced at 168 h (P＜0.05).Compared with group A,the expressions of Bcl-2 and Bax in group B were increased (P＜0.05).The Bax protein level in group B reached the peak at 72 h,and then reduced.Compared with group B,Bax protein level in group C was decreased notably at 72 h and 168 h (P＜0.05),while Bcl-2 was increased significantly (P＜0.05).The Bcl-2 expression in group C increased gradually and reached peak at 168 h,while Bax consistently decreased and descended to the lowest level at 168 h.Conclusion:Xuefu Zhuyu decoction could improve the neurological function in rats with STBI,and its mechanism might be related to the regulation of Bcl-2/Bax expression and the inhibition of brain cell apoptosis.关键词
血府逐瘀汤/重型颅脑损伤/脑细胞凋亡/Bcl-2/BaxKey words
Xuefu Zhuyu decoction/severe traumatic brain injury/brain cell apoptosis/Bcl-2/Bax分类
医药卫生引用本文复制引用
王学敏,安聪,黄宗轩,潘茂华,潘宇政..血府逐瘀汤对重型颅脑损伤大鼠脑细胞凋亡及相关蛋白Bcl-2、Bax的影响[J].广西医科大学学报,2018,35(2):166-169,4.基金项目
国家自然科学基金资助项目(No.81460687) （No.81460687）
广西壮族自治区卫生厅中医药科技专项(No.GZLC14-29) （No.GZLC14-29）
