广西医学2017，Vol.39Issue(12)：1886-1890,5.DOI:10.11675/j.issn.0253-4304.2017.12.34
腺病毒5型早期区1A基因对人肝癌细胞株HepG2辐射敏感性的影响及其作用机制
Effect of adenovirus type 5 early region 1A gene on radiosensitivity of human hepatoma cell line HepG2 and its mechanism
摘要
Abstract
Objective To investigate the effect of adenovirus type 5 early region 1A (E1A) gene on radiosensifivity of human hepatoma cell line HepG2 and its mechanism.Methods HepG2 cells in logarithmic phase were divided into HepG2-E1A group,HepG2-vect group and HepG2 group(HepG2 cells without being transfected).Recombinant plasmid psv-E1A containing E1A gene and plasmid without E1A gene were transfected into HepG2 cells of the HepG2-E1A group and the HepG2-vect group by the vector of polyethylenimine (PEI) respectively.The expressions of E1A mRNA and protein in HepG2 cells,and the cell cycle distribution were detected in the three groups after transfection.The cells in the three groups received radiation of 6 MV-X ray at the doses of 0,1,2,4 and 8 Gy,respectively,then the cell survival rate was assessed in the three group after 72 hours of detection.The cell apoptosis rate was analyzed in the three group after 24 and 72 hours of detection.Results The cells of the HepG2-E1A group expressed E1A mRNA and protein.The cell survival rates of the HepG2-E1A group were lower than those of the other two groups at the radiation dose of 2 Gy and above (P ＜ 0.05).After 24 and 72 hours of radiation at the dose of 1,2,4 and 8 Gy,the cell apoptosis rates of the HepG2-E1A group were higher than those of the other two groups (P ＜ 0.05).In the HepG2-E1A group,the proportion of cells in G2/M phase increased and the proportion of cells in S phase decreased compared to the HepG2-vect group and the HepG2 group (all P ＜0.05).There was no significant difference in the proportion of cells in G1 phase among the three groups (P ＞ 0.05).Conclusion PEI can transfect plasmid psv-E1A gene into hepatoma cells.E1A gene can effectively enhance radiosensitivity of human hepatoma cells,which may be related to its effects on promoting apoptosis,and lead to S phase suppression and G2 phase arrest.关键词
人肝癌细胞/腺病毒5型早期区1A/基因转染/聚乙烯亚胺载体/辐射敏感性Key words
Human hepatoma cell/Adenovirus type 5 early region 1A/Gene transfection/Polyethylenimine/Radiosensitivity分类
医药卫生引用本文复制引用
徐党辉,姚建新,李占峰,潘志尧,姚志峰..腺病毒5型早期区1A基因对人肝癌细胞株HepG2辐射敏感性的影响及其作用机制[J].广西医学,2017,39(12):1886-1890,5.基金项目
江苏省创新团队与领军人才项目(LJ201123) （LJ201123）
