广西医学2017，Vol.39Issue(12)：1895-1899,5.DOI:10.11675/j.issn.0253-4304.2017.12.36
黄芩素对人表皮鳞癌A431细胞线粒体凋亡通路的影响
Effect of baicalein on mitochondrial apoptosis pathway in human epidermal squamous cell carcinoma A431 cells
摘要
Abstract
Objective To investigate the effect of baicalein on mitochondrial apoptosis pathway in human epidermal squamous cell cancer A431 cells.Methods Human epidermal squamous cell cancer A431 cells were divided into blank control group and six groups of baicalein treatment,the baicalein treatment groups were given intervention with the corresponding concentrations of baicalein(10,20,30,40,50 and 60 μg/ml,respectively),and the blank control group was given the equivalent volume of dimethyl sulphoxide(DMSO).The cell inhibition rate was detected in each group after 24,48 and 72 hours of intervention.Then another A431 cells were divided into blank control group and three groups of baicalein treatment,the baicalein treatment groups were given intervention with the corresponding concentrations of baicalein (10,20 and 30 μg/ml,respectively),and the blank control group was given the equivalent volume of DMSO.After 24 hours of intervention,the apoptosis and mitochondrial membrane potential were detected,and the levels of cytoplasmic cytochrome C and apoptosis-inducing factor(AIF) as well as the activities of cysteinyl aspartate-specific proteinase(Caspase)-9 and Caspase-3 were determined.Results Mter 24,48 and 72 hours of intervention,the half maximal inhibitory concentrations(IC50) of baicalein on A431 cells were 82.29 μg/ml,24.67 μg/ml and 18.66 μg/ml,respectively;the total apoptosis rate,the contents of cytoplasmic AIF and cytochrome C,and the activities of Caspase-9 and Caspase-3 in the 10 μg/ml,20 μg/ml and 30 μg/ml baicalein treatment groups were all higher than those in the blank control group (all P ＜ 0.05),and the fluorescence intensity was higher in the baicalein treatment groups compared to the blank control groups (all P ＜ 0.05).The apoptosis rate,the contents of cytoplasmic AIF and cytochrome C,and the activities of Caspase-9 and Caspase-3 positively correlated with the inhibitory concentration of baicalein(P ＜ 0.05),while the fluorescence intensity negatively correlated with the inhibitory concentration of baicalein(P ＜ 0.05).Conclusion Baicalein has significant inhibitory effects on human epidermal squamous cell cancer A431 cells,and the activation of mitochondrial apoptosis pathway may be an important mechanism.关键词
表皮鳞癌/黄芩素/细胞凋亡/线粒体凋亡通路Key words
Epidermal squamous cell cancer/Baicalein/Apoptosis/Mitochondrial apoptosis pathway分类
医药卫生引用本文复制引用
覃静净,樊卓,王丹丹,党云,向桂琼..黄芩素对人表皮鳞癌A431细胞线粒体凋亡通路的影响[J].广西医学,2017,39(12):1895-1899,5.基金项目
陕西省教育厅专项科研计划项目(12JK0754) （12JK0754）
西安医学院附属医院科研基金资助项目(XYFY11-15) （XYFY11-15）
