摘要
Abstract
Objective:To evaluate the association of circulating and tumor tissue miR-146a expressions with the clinicopathological properties and their correlations with prognosis in surgical patients with intrahepatic cholangiocarcinoma (ICC). Methods: 66 ICC patients were enrolled in the study. ICC tissues sample and plasma sample before operation were collected. The expression of miR-146a was determined by quantitative polymerase chain reaction(qPCR). Results:MiR-146a expressions in plasma were positively associated with that in tumor tissue(R=0.342,P=0.005). Tissue miR-146a was negatively correlated with poor differentia-tion (R=-0.253,P=0.041),TNM stage(R=-0.316,P=0.010),as well as ECOG performance(R=-0. 386, P=0.001),while plasma miR-146a was inversely associated with N stage(R=-0.251,P=0.042) and TNM stage (R=-0.282,P=0.022). Kaplan-Meier curves suggested that high expression of tissue miR-146a was correlated with prolonged overall survival (OS) (P<0.001), while plasma miR-146a didn't (P=0. 061). For the purpose of evaluating the prognostic value of tumor tissue or plasma miR-146a for NSCLC pa-tients,univariate Cox regression analysis was performed,which indicated that tissue miR-146a could predict better OS(P<0.001);ECOG high performance(P=0.036),T high stage(P=0.005),N high stage(P=0. 032),M high stage (P=0.006), and TNM high stage (P=0.019) could predict worse OS. Furthermore, multivariate Cox regression analysis revealed that only tissue miR-146a high expression was an independent factor for prolonged OS(P=0.001),while T high stage was a risk factor for OS. Conclusion:The expression level of miR-146a in tumor tissue and plasma is correlated with the clinicopathological characteristics of ICC patients,and miR-146a in tumor tissue may serve as a credible prognostic marker for ICC patients.关键词
肝内胆管癌/肿瘤组织/血浆/miR-146aKey words
Intrahepatic cholangiocarcinoma/Tumor tissue/Plasma/miR-146a
