郑州大学学报（医学版）2018，Vol.53Issue(1)：34-38,5.DOI:10.13705/j.issn.1671-6825.2017.05.123
一株尖孢镰刀菌细胞色素P450氧化酶基因的克隆及生物信息学分析
Molecular cloning and bioinformatics analysis of cytochrome P450 oxidase gene from fusariumoxysporum
摘要
Abstract
Aim:To clone the key enzyme gene,CYP6003A1,which was involved in the reaction of fusariumoxysporum,which could transform drospirenone into an important intermediate(7α,15α-diOH-DHEA).Methods:Primers were designed according to the CYP gene of fusariumoxysporum.The total RNA of fusariumoxysporum was extracted by the method of Trizol extraction from fusariumoxysporum induced by DHEA for 48 h,and the CYP gene of fusariumoxysporum was amplified by PCR and sequenced.The bioinformatics were analyzed.Results:A full-length cDNA fragment of 3 459 bp was successfully amplified.After sequencing,the amino acid sequence of the CYP6003A1 gene was screened by BLAST on NCBI.The results showed that the first 10 genes were similar to CYP6003A1,among them,three were CYP gene,and the others were genes related to CYP gene.The result of bioinformatics analysis showed that the gene fragment encoded 691 amino acids polypeptide.Conclusion:CYP6003A1 gene has been successfully cloned from fusariumoxysporum and analyzed by bioinformatics.关键词
7α,15α-二羟基DHEA/细胞色素P450氧化酶/尖孢镰刀菌/生物信息学Key words
7α, 15α-diOH-DHEA/cytochrome P450 oxidase/fusariumoxysporum/bioinformatics分类
生物科学引用本文复制引用
单丽红,姜冬冬,赵沙沙,黄佳佳,朱丽,刘宏民..一株尖孢镰刀菌细胞色素P450氧化酶基因的克隆及生物信息学分析[J].郑州大学学报（医学版）,2018,53(1):34-38,5.基金项目
国家自然科学基金资助项目(21402178) （21402178）
