郑州大学学报（医学版）2018，Vol.53Issue(1)：53-56,4.DOI:10.13705/j.issn.1671-6825.2017.04.057
沉默精氨酸琥珀酸合成酶对SMMC-7721细胞增殖、凋亡的影响
Effects of argininosuccinate synthetase 1 gene knockdown on proliferation and apoptosis of SMMC-7721
摘要
Abstract
Aim:To investigate the silencing effects of argininosuccinate synthetase 1 (ASS1) gene via shRNA on the proliferation and apoptosis of hepatocellular carcinoma cell line SMMC-7721.Methods:RT-PCR and Western blot were used to examine the expression of ASS1 in 5 different hepatocellular carcinoma cell lines.SMMC-7721 cells were allocated into transfected group(shRNA-ASS1),negative control group (NC group),and blank group(Mock group),and the proliferation and apoptosis of SMMC-7721 cells were examined.Subsequently,Western blot was performed to detect protein expression of ASS1 in the cells.CCK-8 assay was applied to evaluate the cell proliferation on the 3rd day and flow cytometry assay was conducted to estimate cell apoptosis after being transfected for 24 h.Results:The expression of ASS1 mRNA and protein had significant difference among Changliver,SMMC-7721,QGY-7703,HepG2 and HepG3B cells (FmRNA =1 129.140,Fprotein =1 114.240,P ＜ 0.001).SMMC-7721 and HepG3B cells showed high level expression of ASS1 mRNA and protein.CCK-8 assay showed that the proliferation in shRNA-ASS1 group was lower than those in NC group and Mock group(F =7.176,P =0.007).The apoptotic rate in shRNA-ASS1 group was higher than those in NC group and Mock group(F =88.120,P ＜ 0.001).Conclusion:Knockdown of ASS1 by shRNA may inhibit cell proliferation and induce apoptosis of SMMC-7721 cells.关键词
原发性肝癌/精氨酸琥珀酸合成酶/RNA干扰/细胞凋亡Key words
hepatocellular carcinoma/argininosuccinate synthetase 1/RNA interference/cell apoptosis分类
医药卫生引用本文复制引用
李越,何玉婷,李晶晶,陈洁,余炎,胡秋月,阚全程,余祖江..沉默精氨酸琥珀酸合成酶对SMMC-7721细胞增殖、凋亡的影响[J].郑州大学学报（医学版）,2018,53(1):53-56,4.基金项目
河南省科技厅创新人才基金项目(124100510010) （124100510010）
郑州大学第一附属医院青年基金项目(2013年) （2013年）
