郑州大学学报（医学版）2018，Vol.53Issue(1)：56-59,4.DOI:10.13705/j.issn.1671-6825.2017.05.139
LncRNA MEG3对骨肉瘤细胞增殖、侵袭和凋亡的影响
Effect of LncRNA MEG3 on proliferation, invasion and apoptosis in osteosarcoma cells
摘要
Abstract
Aim:To investigate the effect of LncRNA MEG3 (MEG3) on the proliferation,invasion and apoptosis of osteosarcoma cells.Methods:The expression of MEG3 in normal human osteoblast hFOB1.19 and human osteosarcoma cell line MG63 and U-2OS were detected by qRT-PCR.We constructed the recombinant plasmid of pcDNA3.0-MEG3.Then the empty plasmid and the recombinant plasmids were transfected into human osteosarcoma cell lines,the expression of MEG3 in MG63 and U-2OS was detected by qRT-PCR,and cell proliferation,migration and apoptosis were detected by CCK-8 assay,transwell migration assay and flow cytometry assay,respectively.Results:Compared with hFOB1.19,the expressions of MEG3 in osteosarcoma cells were significantly decreased(P ＜0.05).The abilities of proliferation and invasion of MG63 and U-2 OS cells transfected with pcDNA3.0-MEG3 were significantly reduced,and the cell apoptosis was significantly inereased(P ＜0.05).Conclusion:MEG3 is downregulated in osteosarcoma cells,and the overexpression of MEG3 can inhibit the proliferation and invasion,and promote apoptosis of osteosarcoma cells.关键词
MEG3/骨肉瘤细胞/增殖/细胞侵袭/凋亡Key words
MEG3/osteosarcoma cell/proliferation/cell invasion/apoptosis分类
医药卫生引用本文复制引用
刘珂,侯毅,郑稼..LncRNA MEG3对骨肉瘤细胞增殖、侵袭和凋亡的影响[J].郑州大学学报（医学版）,2018,53(1):56-59,4.
