郑州大学学报（医学版）2018，Vol.53Issue(1)：71-75,5.DOI:10.13705/j.issn.1671-6825.2017.04.074
CNRIP1启动子区去甲基化对结肠癌细胞糖酵解及增殖、迁移活性的影响
Effects of CNRIP1 promoter demethylation on glycolysis, proliferation and migration activity of colon cancer cells
摘要
Abstract
Aim:To investigate the effects of cannabinoid receptor interacting protein 1 (CNRIP1) promoter demethylation on glycolysis,proliferation,and migration potential of colon cancer cells.Methods:CNRIP1 promoter methylation in colon cancer cells SW620 was suppressed via treatment with 5-azacytidine(5-azaC),and the CNRIP1 promoter methylation level was determined by bisulfite sequencing PCR;the mRNA and protein expression levels of CNRIP1 as well as key enzymes(HK2,PKM2,LDH-A) of glycolysis pathway were determined by qRT-PCR and Western blot,respectively;lactic acid level in culture medium was determined with biochemistry kit.The proliferative and migration abilities of colon cancer cells were determined with CCK-8 and Transwell cell assays,respectively.Results:Compared with untreated group,CNRIP1 promoter methylation level was decreased significantly in SW620 cells treated with 5-azaC,mRNA and protein expressions of CNRIP1 were increased significantly,mRNA and protein expressions of HK2,PKM2 and LDH-A were depressed significantly,and the proliferative and migration abilities were depressed significantly (P ＜ 0.05).Conclusion:CNRIP1 promoter demethylation could depress the glycolysis pathway,and reduce the proliferative and migration abilities in colon cancer cells.关键词
结肠癌/大麻素受体互作蛋白1/去甲基化/糖酵解/增殖/迁移Key words
colon cancer/cannabinoid receptor interacting protein 1/demethylation/glycolysis/proliferation/migration分类
医药卫生引用本文复制引用
冯馨园,张婷,崔戈,王旗春,钱乙,蔡莹,仰舒静,张梦荧,姚嘉诚..CNRIP1启动子区去甲基化对结肠癌细胞糖酵解及增殖、迁移活性的影响[J].郑州大学学报（医学版）,2018,53(1):71-75,5.基金项目
浙江省自然科学基金项目(LQ16C080001) （LQ16C080001）
浙江省医药卫生研究计划项目(2015KYB372) （2015KYB372）
湖州市科技计划项目(2015GZ14) （2015GZ14）
国家级大学生创新训练项目(201610347015) （201610347015）
