郑州大学学报（医学版）2018，Vol.53Issue(2)：151-154,4.DOI:10.13705/j.issn.1671-6825.2017.07.009
莱菔硫烷对发育早期铅暴露小鼠学习记忆能力及海马组织 β淀粉样蛋白表达的影响
Effect of sulforaphane on spatial learning and memory ability and β-amy-loid protein level in hippocampus from early lead-exposed mice
摘要
Abstract
Aim:To investigate the effect of sulforaphane(SFN) on spatial learning and memory ability and the level of β-amyloid protein(Aβ) in hippocampus from early lead-exposed mice. Methods:In 12 adult Kunming mice,all 8 fe-male mice were randomly divided into two groups by body weight(n=4):the normal group,the lead administrated group. The lead administrated group drank 2 g/L lead acetate aqueous solution for 21 d. The normal group drank lead free deion-ized water. Then,the mice were mated with 1:2 ratio of male to female. After weaning,10 pups of the normal group were randomly selected as the control group who drank lead-freed deionized water for 4 weeks. Meanwhile,the pups of the lead administrated group were divided into 2 groups by weight randomly(10 in each group):lead-exposed group and SFN group. The mice were subjected to gavage daily for 4 weeks. The mice of lead-exposed group were intervened with normal saline, the mice of SFN group were intervened with 25 mg/kg SFN. After intervention,the spatial learning and memory ability was evaluated by the Morris water maze test. Meanwhile,the lead level in the blood was determined by polarography,and the levels of Aβ1-40 and Aβ1-42 in hippocampus were measured by ELISA. Results:In water maze test,the escape latencies of mice in each group were shortened with time,and there was interactive effect between time factor and group factor(P<0.05). Compared with the mice of the control group,the mice of the lead-exposed group performed worse(P<0.05). In addition,both the blood lead level and the Aβ1-40,Aβ1-42 levels in hippocampus of the mice in the lead-exposed group had significant increase compared with the control group(P<0.05). Moreover,the above indexes in the SFN group were significantly decreased compared with the lead-exposed group(P<0.05). Conclusion:SFN could ameliorate cognitive de-cline through decreasing the lead level in the blood and the expression of Aβ1-40,Aβ1-42 of hippocampus in early lead-ex-posed mice.关键词
莱菔硫烷/铅/学习记忆能力/β淀粉样蛋白/阿尔茨海默病/小鼠Key words
sulforaphane/lead/learning and memory ability/β-amyloid protein/Alzheimer's disease/mouse分类
医药卫生引用本文复制引用
孙贝贝,张晓欢,葛惠娜,王俊,韩涵,王腾,李文杰..莱菔硫烷对发育早期铅暴露小鼠学习记忆能力及海马组织 β淀粉样蛋白表达的影响[J].郑州大学学报（医学版）,2018,53(2):151-154,4.基金项目
国家自然科学基金资助项目(81172716) （81172716）
