郑州大学学报（医学版）2018，Vol.53Issue(2)：192-198,7.DOI:10.13705/j.issn.1671-6825.2017.08.080
鼠伤寒沙门菌质粒毒力基因对耐药表型的影响
Influence of spv on antibiotic resistance of Salmonella typhimurium
摘要
Abstract
Aim:To observe the influence of spv on the antibiotic resistance of Salmonella typhimurium isolated from the faeces of the diarrhea children.Methods:The promoter was added to the upstream and the terminator was added to the downstream of the sequence of eGFP published in GeneBank as well as restriction sites was designed,and finally pA-CYC184-eGFP was synthesized using multiple PCR.The spv-complementing plasmid was constructed using pACYC184-eG-FP vector,then electroporated into the spvB and spvC gene-deleted mutant to acquire the corresponding strain, and then drug resistance and the expression of resistance related genes of different strains were examined.Results: PCR and gene sequencing confirmed successful construction of Salmonella typhimurium spvB,spvC gene complemented mutant strain with fluorescence. Low copy plasmid does not lose after 12 generation continuous culture. Compared with the wide type strain and the compensating strain,the MICs of spvB and spvC gene-deleted mutant strain descended original MIC values for pip-eracillin/tazobactam,cefotaxime,cefoxitin,tetracycline,chloramphenicol and ciprofloxacin.The expression of periplasmic protein AcrA in spvB and spvC gene-deleted mutant strain was lower than that of wide type strain and the compensating strain(P<0.05).Conclusion:The spv complementation strain has been constructed successfully on the basis of spvB and spvC-deleted mutant strain,the downregulation of spv can change the antibiotic resistance phenotype of Salmonella typhi-murium,which may due to the downregulation of AcrA.关键词
鼠伤寒沙门菌/沙门菌质粒毒力基因/抗生素耐药/腹泻Key words
Salmonella typhimurium/spv/antibiotic resistance/diarrhea分类
医药卫生引用本文复制引用
朱春晖,王文杏,陈强,陈庆法,吴春雪,刘晓艳,赖华毅,余晓君..鼠伤寒沙门菌质粒毒力基因对耐药表型的影响[J].郑州大学学报（医学版）,2018,53(2):192-198,7.基金项目
江西省自然科学基金资助项目(20132BAB205036) （20132BAB205036）
江西省科技支撑计划课题资助项目(20111BBG70015-2) （20111BBG70015-2）
