郑州大学学报（医学版）2018，Vol.53Issue(2)：198-202,5.DOI:10.13705/j.issn.1671-6825.2017.07.043
低氧对滋养细胞HIF-1α、BNIP3表达及自噬和侵袭能力的影响
Effects of hypoxia on expressions of HIF-1α,BNIP3 and autophagy, in-vasion ability in trophoblasts
摘要
Abstract
Aim:To investigate the effects of hypoxia on the expressions of HIF-1α and BNIP3,and assess the autoph-agy and invasion ability of trophoblasts. Methods: First,HTR-8/SVneo cells were divided into control group,hypoxia group,and autophagy inhibitor 3-MA plus hypoxia group(3-MA+ hypoxia group). After 24 h,Real-time PCR and Western blot were utilized to measure the expression levels of HIF-1α and BNIP3 mRNA and protein in the first 2 groups. Then the expression levels of Beclin1 and LC3 protein as well as the invasion activity of the cells were detected by Western blot and Transwell Matrigel assay. Results:Compared with control group,the expression level of HIF-1α mRNA in hypoxia group had no significant difference(P>0.05);however,the expression levels of BNIP3 mRNA and protein and that of HIF-1α protein in hypoxia group were increased apparently(P<0.05). Compared with control group,the expression levels of Bec-lin 1 and LC3Ⅱ/Ⅰprotein and the invasion ability of trophoblasts increased(P<0.05);compared with hypoxia group,the expression levels of Beclin 1 and LC3Ⅱ/Ⅰ protein decreased,and the invasion ability was reduced in 3-MA +hypoxia group(P<0.05). Conclusion: Hypoxia could induce autophagy and enhance the invasion ability of trophoblasts via the HIF-1α/BNIP3 pathway;the invasion ability of trophoblasts under hypoxia is decreased in the presence of 3-MA.关键词
缺氧/滋养细胞/自噬/HIF-1αKey words
hypoxia/trophoblast/autophagy/HIF-1α分类
医药卫生引用本文复制引用
闫广伟,丁燕子,邢金芳,代延朋,杜红梅,袁恩武..低氧对滋养细胞HIF-1α、BNIP3表达及自噬和侵袭能力的影响[J].郑州大学学报（医学版）,2018,53(2):198-202,5.基金项目
河南省科技厅创新项目(20140269) （20140269）
