郑州大学学报（医学版）2018，Vol.53Issue(2)：206-209,4.DOI:10.13705/j.issn.1671-6825.2017.05.125
二氧化铈纳米颗粒预处理对大鼠缺血再灌注损伤心肌细胞Nrf2信号通路的影响
Influence of pretreatment of nanoceria on Nrf2 signaling pathway in rat cardiomyocytes with ischemia reperfusion injury
摘要
Abstract
Aim: To research the influence of pretreatment of nanoceria on the expression of nuclear factor erythroid-related factor 2(Nrf2) in cardiomyocytes of rats with ischemia-reperfusion(IR) injury. Methods: Fifty adult healthy rats were randomly allocated into 5 groups,and 10 in each group:the sham operation group, model group, and 3 pretreatment groups. The rats in model group and 3 pretreatment groups were made myocardial IR model(ischemia for 45 min and reper-fusion for 120 min). Pretreatment group 1,2,3 were injected through the tail vein with 1-10,10-25,and 50 nm nanoc-eria suspension(2 mL/kg)24 h before the operation. After the reperfusion,the expressions of Nrf2,NQO1 and HO-1 mR-NA and the expression of Nrf2 protein in the nucleus in IR cardiomyocytes were detected by qRT-PCR and Western blot. Results:The expressions of NQO1,HO-1 mRNA,and Nrf2 protein in the nuclei in the model group was significantly high-er than those in the sham operation group(P<0.05), but the expression of Nrf2 mRNA had no significant change(P>0.05).Compared with those of the model group,the expressions of Nrf2,NQO1,HO-1 mRNA and Nrf2 protein in the nu-clei in the 3 pretreatment groups increased(P<0.05),and the changes of the above indexes in pretreatment group 2 were the most significant(P<0.05).Conclusion:The pretreatment with nanoceria may activate the Nrf2 signaling pathway,so as to achieve myocardial protection.关键词
二氧化铈纳米颗粒/Nrf2信号通路/心肌缺血再灌注损伤/大鼠Key words
nanoceria/Nrf2 signaling pathway/myocardial ischemia reperfusion injury/rat分类
医药卫生引用本文复制引用
黄真锋,法宪恩,朱方涛,王宏山,高成山,柳兵..二氧化铈纳米颗粒预处理对大鼠缺血再灌注损伤心肌细胞Nrf2信号通路的影响[J].郑州大学学报（医学版）,2018,53(2):206-209,4.基金项目
国家自然科学基金资助项目(81241003) （81241003）
河南省科技创新杰出人才计划资助项目(104200510007) （104200510007）
郑州市科技创新人才培育计划项目领军人才基金资助项目(10LJRC175) （10LJRC175）
