摘要
Abstract
Aim:To study the effects of inhibiting Akt signaling pathway on the growth,cell cycle and apoptosis of fi-broblasts in pathological scar. Methods: Human pathological scar fibroblasts were isolated and cultured in vitro. After treatment with different concentrations of Akt signaling pathway inhibitor LY294002, cell proliferation was detected by MTT,and IC50was calculated. After treatment by IC50concentration of LY294002,cell cycle and apoptosis were detected by flow cytometry, and the levels of Cleaved Caspase-3, Cleaved Caspase-9, Akt, and p-Akt were detected by Western blot.Results: The viability decreased significantly after LY294002 treatment with different concentrations(P<0.001), and the IC50was 30 mg/L. After treatment by 30 mg/L LY294002,compared with 0 mg/L group,the G0/G1 phase cells were significantly increased(P<0.001), the apoptosis rate was elevated, the levels of Cleaved Caspase-3 and Cleaved Caspase-9 were significantly higher,while the level of p-Akt was significantly lower(P<0.001).Conclusion: Inhibition Akt signaling pathway could inhibit fibroblasts growth in pathological scar, block cell cycle, promote cell apoptosis, and promote the activation of Caspase-3 and Caspase-9.关键词
Akt信号通路/病理性瘢痕成纤维细胞/细胞周期/凋亡Key words
Akt signaling pathway/pathological scar fibroblast/cell cycle/apoptosis分类
医药卫生
