河南农业科学2018，Vol.47Issue(2)：91-97,7.DOI:10.15933/j.cnki.1004-3268.2018.02.018
169份辣椒种质资源的遗传多样性分析
Genetic Diversity Analysis of 169 Accessions of Capsicum
摘要
Abstract
In this study 152 pairs of SSR primers were designed based on the whole genome coding region sequence of pepper. The different species of Capsicum were used to screen 152 pairs of primers,and among them 17 pairs of SSR polymorphic primers showing clear bands and good stability were obtained. The genetic diversity of different species of pepper germplasm was studied by these primers.POPGENE32 and Phylip softwares were used to analyze the experimental data.The results showed that 17 pairs of SSR primers amplified 46 polymorphic bands,with an average of 2.71 loci amplified by each pair of primers. The mean value of allele number(Na),effective allele number(Ne),expected heterozygosity(He), observed heterozygosity(Ho),Shannon-Weaver index(I) and polymorphism information content(PIC) were 2.176 5,1.629 1,0.360 2,0.834 7,0.549 6 and 0.310 6 respectively,indicating that pepper genetic information was very rich.The 169 accessions of Capsicum were analyzed using UPGMA method, and clustered into seven branches.关键词
辣椒/SSR标记/遗传多样性/聚类分析Key words
Pepper/SSR marker/Genetic diversity/Clustering analysis分类
农业科技引用本文复制引用
李艳,赵红星,王勇,姜俊,孟祥锋,魏小春,李金玲..169份辣椒种质资源的遗传多样性分析[J].河南农业科学,2018,47(2):91-97,7.基金项目
河南省基础与前沿技术研究计划项目(162300410155) （162300410155）
国家大宗蔬菜产业技术体系项目(CARS-23-G18) （CARS-23-G18）
河南省大宗蔬菜产业技术体系项目(Z2010-03-06) （Z2010-03-06）
河南省辣椒核心种质创制及分子育种创新型科技团队项目(C20150054) （C20150054）
