海洋科学2017，Vol.41Issue(11)：1-7,7.DOI:10.11759/hykx20170715001
4种壳色蛤仔TYR基因的表达特性研究
TYR gene expression profiling in four shell color strains of Manila clam (Ruditapes philippinarum)
摘要
Abstract
In order to understand the relationship between the pigment control gene and shell color, the expression characteristics of tyrosinase (TYR) in four selected strains of Manila clam (Orange, Zebra, White zebra, and White) were analyzed in relative quantification PCR. The results from gill, mantle, adductor muscle, gonad, visceral mass, siphon and palp tissue showed that the tyrosinase gene expression in mantle was higher than other tissues and least expressed in adductor muscle. All four shell color strains of Manila clam have different levels in the expression of tyrosinase gene. The high expression of TYR2, TYR3, TYR6 and TYR9 in the white zebra, while the white clam was significantly different(P<0.05).TYR2,TYR6 and TYR10 were the highest expression in Orange strain,suggesting it maybe related to the formation of orange shell color.While TYR11 was the highest expression in zebra clam,and was significantly different from white clams and white zebra clams (P < 0.05), presumably related to the formation of background color. Phylogenetic analysis showed that the homology of TYR3 to Pinctada martensii was 64%. The highest homology of TYR10 to Octopus bimaculoides was 53%. It has recently been involved in the evolution of Crassostrea giga.关键词
菲律宾蛤仔(Ruditapes philippinarum)/酪氨酸酶基因/相对荧光定量聚合酶链式反应(Polymerase Chain Reaction,PCR)/黑色素Key words
Ruditapes philippinarum/tyrosinase/relative quantification RT-PCR/melanin分类
农业科技引用本文复制引用
姜力文,聂鸿涛,李东东,霍忠明,李妹妍,闫喜武..4种壳色蛤仔TYR基因的表达特性研究[J].海洋科学,2017,41(11):1-7,7.基金项目
现代农业产业技术体系建设专项资金(CARS-49) （CARS-49）
国家自然科学基金(31302183) （31302183）
辽宁省高等学校杰出青年学者成长计划(LJQ2014076).The Earmarked Found for Modern Agro-industry Technology Research System,No.CARS-49 （LJQ2014076）
National Natural Science Fund,No.31302183 （）
The Program for Liaoning Excellent Talents in University,No.LJQ2014076.[ （）
