海洋科学2017，Vol.41Issue(11)：45-54,10.DOI:10.11759/hykx20170626001
肠道细菌对厚壳贻贝稚贝附着的作用研究
Effects of gut bacteria on the settlement of spats of Mytilus coruscus
摘要
Abstract
Mytilus coruscus is an important marine commercial mollusc in southeast coastal areas of China due to its high nutritional and dietary value, while overfishing has resulted in the increasing scarcity of wild mussel re-sources. Thus, improving the technique of artificial culture in this species is necessary. Various biofilms have been well-recognized to play essential roles in the settlement of larvae and juveniles of many species of invertebrates. However, the relationship between gut bacterial biofilm and settlement of invertebrates is poorly characterized. In this study,to understand settlement of the mussel M.coruscus in response to the different bacterial strains isolated from the gut of M. coruscus adults, the interaction between bacterial biofilms and settlement was investigated. In addition, the phylogenetic tree derived from the selected 16S rDNA sequence was constructed and the genetic dis-tance was calculated. The results showed that the density of monospecific bacterial biofilms increased with the ini-tial incubation density and all test bacterial biofilms showed significantly inducing activity on settlement of M. coruscus spats.Among the 10 bacterial species,the biofilm of Paracoccus sp.1 induced the highest percentage of settlement of 61%, and biofilm thickness relatively was thick and intensive. There was signification correlation between the settlement and the cell densities of biofilms of Roseovarius sp.1,Winogradskyella sp.1,Tenacibacu-lum sp.4,Nonlabens sp.1 and Bacillus sp.5.The inducing activity of individual bacterial isolates was not corre-lated with their phylogenetic relationship. Thus, gut bacteria can promote the settlement of M. coruscus, and may have a positive effect on the growth and development of M.coruscus.The findings will provide a novel insight into understanding the relationship between gut bacteria and the growth and development of M. coruscus, and also are helpful for enhancing the artificial culture of the species.关键词
厚壳贻贝/稚贝/附着/肠道细菌/微生物被膜Key words
Mytilus coruscus/spat/settlement/gut bacteria/biofilm分类
生物科学引用本文复制引用
杨娜,梁箫,彭莉华,沈和定,杨金龙..肠道细菌对厚壳贻贝稚贝附着的作用研究[J].海洋科学,2017,41(11):45-54,10.基金项目
国家自然科学基金(41476131) （41476131）
上海高校水产高峰学科建设项目(水产科学).National Natural Science Foundation of China,No.41476131 （水产科学）
The Peak Discipline Program for Fisheries from the Shanghai Municipal Government. （）
