海洋科学2017，Vol.41Issue(11)：107-112,6.DOI:10.11759/hykx20170727001
基于CO1基因的渔山列岛厚壳贻贝遗传资源评估
Genetic resources assessment of mussel Mytilus coruscus based on CO1 gene in Yushan Island
摘要
Abstract
Partial nucleotide sequences of mitochondrial DNA CO1gene were sequenced and analyzed to estimate the genetic diversity and genetic structure of Mytiluscoruscus populations in Yushan island,Zhangzi island of Da-lian, Nanji island, Nanhuangcheng islands of Shandong, and Shengsi island of Zhoushan populations. In the mtDNA, homologous fragments of CO1 gene were detected in 39 polymorphic sites, including 39 parsimony informative sites, no single mutation, 40 haplotypes were identified. The results of the genetic diversity level ranging from high to low were as follows: Yushan, Shandong, Dalian, Zhoushan, and Nanji populations. The haplotype diversity ranging from high to low was as follows: Shandong, Yushan, Nanji, Dalian, and Zhoushan populations. Three ge-nealogical clades(A,B,C)were checked in the Mytiluscoruscus populations in NJ and MST analyses.Genealogical clade-A was found to have 15 haplotypes, 13 in genealogical clade-B, and 2 in genealogical-C (all of them are hap-lotypes of the Yushan Island). The Yushan island's haplotypes were disturbed in three genealogical clades, the ge-netic distances among the clades ranged from 0.0099 to 0.0268. The genetic differentiation of Yushan population was higher than that of the other four populations.关键词
CO1基因/遗传资源/渔山列岛/厚壳贻贝(Mytiluscoruscus)/单倍型Key words
CO1 gene/Genetic resources/Yushan Island/Mytiluscoruscus/haplotype分类
生物科学引用本文复制引用
袁文斌,李长保,焦海峰,林志华,包永波..基于CO1基因的渔山列岛厚壳贻贝遗传资源评估[J].海洋科学,2017,41(11):107-112,6.基金项目
浙江省公益性项目(2016C33090) （2016C33090）
宁波市农业科技攻关项目(2014C10018).Zhejiang Province Public Technology Applied Research Projects,No.2016C33090 （2014C10018）
Ningbo Agricultural Science and Technology Research Project,NO.2014C10018. （）
