解放军医学杂志2018，Vol.43Issue(1)：17-22,6.DOI:10.11855/j.issn.0577-7402.2018.01.04
基于基质辅助激光解吸电离飞行时间质谱系统的血流感染致病菌早期检测
Early detection of the bloodstream infection bacteria based on MALDI-TOF MS
张可昕 1胡翀 2陈琛 2何赏 2杨明 2王成彬1
作者信息
- 1. 325000浙江温州 温州医科大学检验医学院/生命科学学院
- 2. 100853北京 解放军总医院临床检验科
- 折叠
摘要
Abstract
Objective To establish a clinical method for early detection of the bloodstream infection bacteria based on matrix-assisted laser ionization time of flight mass spectrometry (MALDI-TOF MS).Methods After consulting the Laboratory Information System statistics and domestic related literature,We chose 10 kinds of bacteria (Escherichia coli.,Pseudomonas aeruginosa,Acinetobacter baumannii,Klebsiella pneumoniae,Enterococcus faecium,Enterococcus faecalis,Staphylococcus aureus and Staphylococcus epidermidis,Proteus mirabilis,Streptococcus pneumoniae) as target.The MALDI-TOF MS was established using simulated bacterial infection blood samples.From March to May 2017,33 blood samples of suspected sepsis patients from Emergency Department,General Hospital of PLA,were tested.Results The MALDI-TOF MS whose detecting sensitivity was 100CFU/ml had the same negative detection rate with the blood culture (27cases/27cases,100％).Besides the 2 samples of Morganella morganii infection and Staphylococcus hominis infection were out of the range,the results of the remaining 4 positive samples were consistent (100％).Conclusion Compared to the blood culture and biochemical identification,MALDI-TOF MS can rapidly detect 10 kinds of bloodstream infection bacteria with high sensitivity and high accuracy.关键词
基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)/细菌性血流感染/早期诊断Key words
Blood stream infection bacteria/Early detection/High-throughput detection分类
医药卫生引用本文复制引用
张可昕,胡翀,陈琛,何赏,杨明,王成彬..基于基质辅助激光解吸电离飞行时间质谱系统的血流感染致病菌早期检测[J].解放军医学杂志,2018,43(1):17-22,6.
