解放军医学杂志2018，Vol.43Issue(2)：96-100,5.DOI:10.11855/j.issn.0577-7402.2018.02.02
麒麟菜多肽对血小板聚集及角叉菜胶诱导的小鼠尾部血栓形成的影响
Effects of Eucheuma peptides on platelet aggregation and mouse tail thrombosis induced by carrageenan
摘要
Abstract
Objective This study is designed to investigate the effect of the peptide from Eucheuma on platelet aggregation and mouse tail thrombosis induced by carrageenan.Methods The peptide was purified from Eucheuma using sephdexG-50 gel chromatography and HPLC.The inhibitory effects of peptide on platelet aggregation induced by adenosine diphosphate (ADP)/thrombin were detected by light transmittance aggregometry.The platelet were treated with normal saline or Eucheuma peptides (2.5,5.0,10.0μg/ml).The platelet aggregation was detected by FLASH continuous spectral fluorescence microplate reader,and the maximum platelet aggregation rate (MAR) was calculated.The peptide (15,30,60mg/kg) and 100mg/kg clopidogrel were intraperitoneal injected into mice for 7 days to confirm the anti-thrombosis activity of the peptide in carrageenan-induced mouse tail thrombosis model.Results The platelet aggregation can be inhibited by the peptide.Comparing the maximum platelet aggregation rate induced by ADP,the results showed that control group was 100％,2.5μg/ml peptide group was 61.84％,5.0μg/ml peptide group was 71.04％ and 10.0μg/ml peptide was 60.95％.The maximum platelet aggregation rate induced by thrombin was also tested.The results showed that control group was 100％,2.5μg/ml peptide group was 75.34％,5.0μg/ml peptide group was 69.96％ and 10.0μg/ml peptide group was 73.74％.The thrombosis rate (2.5μg/ml peptide group was 36.89％,5.0μg/ml peptide group was 17.25％,10.0μg/ml peptide group was 22.99％,and 100mg/kg clopidogrel group was 18.96％,respectively) and the length of thrombus in mice can be reduced by the peptide (15-60mg/kg).Conclusions The carrageenan induced tail thrombosis in mice can be inhibited by the peptide of Eucheuma.It is suggested that the peptide of Eucheuma is a potential compound in anti-thrombosis.关键词
麒麟菜/多肽/角叉菜胶/血栓Key words
Eucheuma/peptide/carrageenan/thrombosis分类
医药卫生引用本文复制引用
余华军,伍俊,吴尚,李慧敏,黄慧,张海涛..麒麟菜多肽对血小板聚集及角叉菜胶诱导的小鼠尾部血栓形成的影响[J].解放军医学杂志,2018,43(2):96-100,5.基金项目
国家自然科学基金(81273549) （81273549）
广东医科大学科研基金(2XJ14027)This work was supported by the National Natural Science Foundation of china (81273549) and Guangdong Science and Technology Project (2XJ14027) （2XJ14027）
