解放军医学杂志2018，Vol.43Issue(2)：120-125,6.DOI:10.11855/j.issn.0577-7402.2018.02.06
原花青素对人脐静脉内皮细胞功能及miR-221表达的影响
Effect of procyanidine on the function and expression of miR-221 in human umbilical vein endothelial cells
摘要
Abstract
Objective To study the effect ofprocyanidine (PC) on the proliferation and migration of human umbilical vein endothelial cells (HUVECs),determine the expression changes of miR-221,and to investigate the mechanism involved.Methods HUVECs were cultured in vitro and treated with PC (5,25,50,75,100μg/ml) for 24 hours,and a PC concentration of 50μg/ml was screened by CCK-8 assay for the follow up experiment,then the cell proliferation activity was detected by the wound healing assay.The expression of miR-221 in HUVECs was detected by real-time quantitative PCR;MiR-221 target genes were predicted in miRWalk database,and the target genes were analyzed by GO and KEGG pathway.Results Compared with the control group,the HUVECs treated with PC for 24h,their proliferation activity in PC 5μg/ml group did not change obviously (P＞0.05),and in PC 25,50,75 and 100μg/ml groups decreased in a concentration dependent manner (P＜0.01).Compared with the control group,the migration ability of PC 50μg/ml group decreased markedly (P＜0.01).Compared with the control group,the expression of miR-221 increased after treatment with PC 50μg/ml (P＜0.01).Go analysis indicated that the target genes of miR-221 were mainly related to cell proliferation,migration,gene translation and so on.The target genes related to cell proliferation and migration were ADAM17,KIT,PDGFD and so on.KEGG pathway analysis showed that the target genes of miR-221 enriched 5 signal pathways,such as FoxO,PI3K-Akt and so on.Conclusions Low concentration of PC has no effect on the proliferation activity of HUVECs.A certain concentration of PC can inhibit the proliferation and migration of HUVECs,of which the mechanism may be involved with the up-regulation of miR-221 and FoxO,PI3K-Akt and other signaling pathways.关键词
原花青素/人脐静脉内皮细胞/miR-221Key words
procyanidine/HUVECs/miR-221分类
医药卫生引用本文复制引用
邹燕,吴斐,季昳弛,何雪梅,周翔宇..原花青素对人脐静脉内皮细胞功能及miR-221表达的影响[J].解放军医学杂志,2018,43(2):120-125,6.基金项目
泸州市人民政府-西南医科大学科技战略合作项目(2017LZXNYD-T10)This work was supported by the Luzhou-Southwest Medical University Joint Research (2017LZXNYD-T10) （2017LZXNYD-T10）
