生物质化学工程2018，Vol.52Issue(2)：29-34,6.DOI:10.3969/j.issn.1673-5854.2018.02.006
两种木质素对纤维素酶水解的影响机制
Effect of Two Kinds of Lignin on Enzymatic Hydrolysis of Cellulose
摘要
Abstract
In order to investigate the effect of lignin on the enzymatic hydrolysis of cellulose, the ethanol organosolv lignins (EOL-B)and milled wood lignin(MWL-B)were extracted from bitter bamboo and added to the microcrystalline cellulose for enzymatic digestibility and adsorption.The results showed that the addition of MWL-B(8 g/L)decreased the 72 h hydrolysis yields of glucose from 51.34%to 46.06%.But the addition of EOL-B(8 g/L)significantly improved the 72 h hydrolysis yields of glucose from 51.34%to 61.06%.Langmuir adsorption isotherms showed that EOL-B had lower binding ability and maximum adsorption capacity to the cellulase enzymes than MWL-B,which led to less non-productive binding.And FT-IR and 13C NMR spectra showed that the condensed structure of C—C of lignin reduced and the β-O-4′linkages cracked in the ethanol organosolv process,which resulted in increasing the hydrophilicity of lignin,blocking the combination of lignin and the hydrophobic amino acid of cellulose,decreasing the adsorption of cellulose,increasing the cellulose concentration around the cellulose substrate and promoting the effect of enzymatic hydrolysis.关键词
苦竹/乙醇木质素/磨木木质素/酶水解/亲水性基团Key words
bitter bamboo/ethanol organosolv lignins/milled wood lignin/enzymatic hydrolysis/hydrophilic group分类
化学化工引用本文复制引用
吴凯,应文俊,郑志锋,史正军,杨海艳,杨静..两种木质素对纤维素酶水解的影响机制[J].生物质化学工程,2018,52(2):29-34,6.基金项目
云南省农业基础研究联合专项项目(2017FG001-025) （2017FG001-025）
云南省教育厅科学研究基金项目(2017YJS092) （2017YJS092）
国家自然科学基金资助项目(3176030250) （3176030250）
