南京农业大学学报2018，Vol.41Issue(2)：256-265,10.DOI:10.7685/jnau.201706002
铜胁迫下葡萄植株的生理响应和铜转运蛋白基因VvCTR1的克隆及分析
Physiological responses to copper stress in grape plants and cloning and analysis of copper transport VvCTR1 gene
摘要
Abstract
[Objectives]This study aims to analyze the effects of heavy metal copper on the growth and development of grape plants, and provided theoretical basis for revealing the mechanism of damage by heavy metal copper to plants. [Methods]The excess CuSO4 and the mixture of CuSO4and its chelating agent were applied on grape seedlings,the root and leaf development,leaf chlorophyll content,and the distribution of copper in plants were analyzed;copper transport VvCTR1 gene was isolated,and its expression levels were analyzed during fruit development. Its structure and homology among species were analyzed,and its expression levels were analyzed under the copper stress in roots,stems and leaves. [Results]Compared to the control,excess CuSO4,the mixture of CuSO4 and citric acid or EDTA inhibited the development of the number and length of new roots in grape,promoted the degradation of chlorophyll and leaf rate drop,and increased the production of ethylene and the polygalacturonase and cellulase activities in petiole base. Especially there were obvious damage in mixture of CuSO4and EDTA treatment. The content of Cu2+in leaves,stems and roots from small to large was the morphologically up to down of the stems and leaves and old roots to new roots in turn. And the content of copper ion in CuSO4treated plants was higher than control,and showed an increase with the extension of time,especially the mixture of CuSO4and citric acid or EDTA treatment had more copper ions;there was no intron in copper transport VvCTR1 gene,and it contained three transmembrane regions of TMD1-3,which N contained a methionine rich region. Its expression decreased gradually with the fruit development,and the expression of VvCTR1 gene was enhanced under copper both in roots and in different positions of stems and leaves. [Conclusions]Excessive copper can cause damage to grape plants,especially when combined with Cu2+chelate citric acid or EDTA,which can make more Cu2+enter the plant and ultimately cause grape plant death.关键词
铜/葡萄/生理性状/基因表达Key words
opper/grape/physiological character/gene expression分类
农业科技引用本文复制引用
刘众杰,李傲,崔梦杰,许瀛之,上官凌飞,贾海锋,王晨,房经贵..铜胁迫下葡萄植株的生理响应和铜转运蛋白基因VvCTR1的克隆及分析[J].南京农业大学学报,2018,41(2):256-265,10.基金项目
国家自然科学基金项目(31672131) （31672131）
国家重点实验室开放课题项目(ZW2014009) （ZW2014009）
中国博士后特别资助项目(2014T770533) （2014T770533）
南京农业大学校青年基金项目(KJ2013013) （KJ2013013）
中央高校基本科研业务费专项资金(KJQN201541) （KJQN201541）
