南京农业大学学报2018，Vol.41Issue(2)：364-369,6.DOI:10.7685/jnau.201703030
明胶对鸡胚盘细胞的增殖和多功能性的影响
Effects of gelatin on proliferation and pluripotency of chicken blastodermal cells
摘要
Abstract
[Objectives]This paper aims to overcome the difficulties of adherence of chicken blastodermal cells(cBC)and to establish a new culture system for chicken blastodermal cells by using E8 medium.[Methods]By using 10 g·L-1gelatin-coated and human recom-binant vitronectin(hVTN)-coated wells,we compared with each other in the adherent rate and maintaining pluripotency of chicken blastodermal cells. [Results]Treated by 10 g·L-1gelatin on the wells,chicken blastodermal cells could be pasted to the wells within 12 hours.5-ethynyl-2′-deox-youridine(EdU)positive cells in the 10 g·L-1gelatin treatment group were significantly higher than those in the control(P<0.01),and could inhibit the mRNA expression of P21 and Fas(P<0.05),and thus promote cell proliferation,and promote the expression of multifunctional genes(Nanog,PouV)to maintain pluripotency of cBC. [Conclusions]10 g·L-1gelatin can be used as a new embedding reagent for culturing cBC. And compared with the hVTN,treated by 10 g·L-1gelatin on the wells has higher cost performance and higher application value.关键词
鸡胚盘细胞/明胶/增殖/多功能性Key words
chicken blastodermal cells(cBC)/gelatin/proliferation/pluripotency分类
农业科技引用本文复制引用
李荣阳,唐小川,徐世永,陈清,吴望军,刘红林..明胶对鸡胚盘细胞的增殖和多功能性的影响[J].南京农业大学学报,2018,41(2):364-369,6.基金项目
"南京创业人才321计划"项目 （）
