南京农业大学学报2018，Vol.41Issue(2)：382-388,7.DOI:10.7685/jnau.201708004
连续施用生物有机肥对草地生物量及土壤微生物区系的影响
Effect of consecutive biofertilizer application on aboveground biomass and management of soil microflora in grassland
摘要
Abstract
[Objectives]Our study aims to understand the promotion effect of biofertilizer on grassland biomass,and demonstrate the potential soil microecological regulation mechanisms,providing an important theoretical basis for constructing high-yield soil rhizo-sphere microflora. [Methods]We conducted a 2-year in situ fertilization experiment in meadow steppe grasslands in Hulunber,Inner Mongolia,China to evaluate the impacts of different fertilization regime(non-amend fertilization,organic fertilizer,and biofertilizer)on grassland biomass,and the high-throughput sequencing was employed to monitor the potential variation of soil microflora. [Results] The aboveground biomass significantly increased in plots fertilized with biofertilizer(BOF,9 000 kg·hm-2)compared with plots that received organic fertilizer(OF,9 000 kg·hm-2)or no fertilizer(CK). Nonmetric multidimensional scaling(NMDS)revealed that bacterial community structure was relatively similar in the soils of different treatments,while soil fungal community structure was significantly separated by different fertilization regime. BOF application increased the relative abundances of Archaeorhizomyces and Trichoderma,but decreased that of Ophiosphaerella. The correlations between soil properties and microbial genera showed that a higher soil available P might be beneficial to the aboveground biomass by increasing the abundances of Archaeorhizomyces and Trichoderma and decreasing Ophiosphaerella in the soil. [Conclusions] Consecutive biofertilizer application can improve the aboveground biomass by manipulating the soil microbial community structure and composition in grasslands.关键词
生物有机肥/草地生物量/土壤理化性质/高通量测序/土壤微生物区系/皮尔森相关指数Key words
biofertilizer/grassland biomass/soil properties/high-throughput sequencing/soil microflora/Pearson correlation coefficients分类
农业科技引用本文复制引用
张风革,霍云倩,孙艺,肖燕,杨高文,张英俊..连续施用生物有机肥对草地生物量及土壤微生物区系的影响[J].南京农业大学学报,2018,41(2):382-388,7.基金项目
国家自然科学基金青年基金项目(31602006) （31602006）
中国博士后基金面上项目(2015M581815) （2015M581815）
