青岛大学医学院学报2017，Vol.53Issue(5)：505-507,510,4.DOI:10.13361/j.qdyxy.201705001
miR-182-5p抑制剂对5-FU耐药胃癌细胞株化疗的增敏作用
SENSITIZING EFFECT OF MIR-182-5P INHIBITOR ON 5-FU-RESISTANT GASTRIC CANCER CELL LINE IN CHEMOTHERAPY
摘要
Abstract
Objective To investigate the effects of miR-182-5p inhibitor combined with 5-fluorouracil (5-FU) on the proliferation,apoptosis,and migration of 5-FU-resistant human gastric cancer cell line (SGC7901/5-FU) and the sensitizing effect of miR-182-5p inhibitor on gastric cancer cells in 5-FU chemotherapy.Methods SGC7901/5-FU cells were divided into miR-182-5p oligonucleotide negative control group (group A),miR-182-5p inhibitor group (group B),5-FU group (group C),and miR-182-5p inhibitor combined with 5-FU group (group D).The proliferation,apoptosis,and migration of SGC7901/5-FU cells in each group were measured by MTT assay,flow cytometry,and Transwell assay,respectively.Results There were significant differences in survival rate,apoptosis rate,and migration rate of SGC7901/5-FU cells between group D and groups A,B,and C (F =107.38-490.53,q=4.23-48.60,P＜0.05).Conclusion A combination of miR-182-5p inhibitor and 5-FU significantly reduces the proliferation and migration of SGC7901/5-FU cells,promotes cell apoptosis,and increases the sensitivity of SGC7901/5-FU cells to 5-FU chemotherapy.关键词
微RNAs/胃肿瘤/氟胞嘧啶/增敏作用Key words
microRNAs/stomach neoplasms/flucytosine/sensitization分类
医药卫生引用本文复制引用
史俊英,牟晓峰,王晔,刘成玉..miR-182-5p抑制剂对5-FU耐药胃癌细胞株化疗的增敏作用[J].青岛大学医学院学报,2017,53(5):505-507,510,4.基金项目
国家自然科学基金资助项目(81670822,81370990) （81670822,81370990）
