青岛大学医学院学报2017，Vol.53Issue(5)：600-603,605,5.DOI:10.13361/j.qdyxy.201705028
鱼胶原蛋白肽-透明质酸水凝胶制备及其生物相容性
PREPARATION OF FISH COLLEGEN PEPTIDE-HYALURONIC ACID HYDROGEL AND ITS BIOCOMPATIBILITY
摘要
Abstract
Objective To investigate the preparation of pollock skin collagen peptide-hyaluronic acid hydrogel and its influence on biological characteristics of human umbilical cord mesenchymal stem cells cultured in vitro.Methods Methacrylic anhydride,as well as fish collagen peptide and hyaluronic acid,was used to synthesize photocrosslinkable material,and human umbilical cord mesenchymal stem cells were embedded in the hydrogel for three-dimensional culture.Fluorescein diacetate staining was used to observe cell viability in the hydrogel,EdU staining and MTT assay were used to measure cell proliferation,and immunofluorescent staining was used to observe cell morphology.Results The human umbilical cord mesenchymal stem cells survived and grew well in the gel.Compared with those with adherent growth,the cells in three-dimensional culture had a spherical shape,a steady growth rate,and persistent proliferation.Conclusion Fish collagen peptide-hyaluronic acid hydrogel is suitable for cell embedding and has the potential for wide application in various fields of tissue engineering.关键词
胶原/透明质酸/水凝胶/细胞增殖/组织工程Key words
collagen/hyaluronic scid/hydrogel/cell proliferation/tissue engineering分类
药学引用本文复制引用
魏志君,施春英,程连强,苏威,季爱玉..鱼胶原蛋白肽-透明质酸水凝胶制备及其生物相容性[J].青岛大学医学院学报,2017,53(5):600-603,605,5.基金项目
青岛市科技转化计划(科技惠民专项)(15-9-2-76-nsh) （科技惠民专项）
