四川中医2018，Vol.36Issue(2)：56-60,5.
肺为水之上源理论与湿阻中焦证致肺水通道蛋白变化的关联性研究
Study on Relevance of the theory of lung as the source of water and syndrome of dampness in middle Jiao caused the changes of Aquaporins in the lung
摘要
Abstract
Obhective:There are many factors of the source of syndrome of dampness in middle Jiao,the spleen deficiency is the main cause.The lung and spleen indistribution and excretion of body fluid is coordinated in the lung is based on lung as the source of waterunder the guidance of the theory,experimental study on the effect of the expression of syndrome of dampness in middleJiaoof Aquaporins in the lung,to reveal the lung and spleen in water metabolism and pathological correlation.Methods:Build a model of the syndrome of dampness in middle Jiao,simulating the three big etiological factors of retention of water and dampness:i.e.the functioning declination of spleen and stomach by exterior dampness luxus,interior dampness caused by intemperant diet,or stagnation of Qi caused by emotional depression.Measure the levels of AQP1,AQP4and AQP8in lung tissue and the changes of Chinese medicine after intervention by enzyme-linkedimmunosorbent assay (Elisa).Results:The expression of AQP1and AQP4was decreased in the model before and after modeling,but this difference is no significant.The expression of AQP8was significantly decreased in the model before and after modeling.The expression of AQP1 in all groups showed an upward trend.The expression of AQP8decreased obviously in the high dose and low dose groups of BuhuanjinzhengqiSan.The syndrome of dampness in middle Jiao,expression of Aquaporin in the lung is changed,and the prescription of Traditional Chinese Medicine forsyndrome of dampness in middle Jiaohas significant curative effect,have certain effect on the changes.Conclusion:There is a certain correlation between lung and spleen in water metabolism.Expression of Aquaporins in the lung,the change of the expression in syndrome of dampness in middle Jiao and the change of the expression in the intervention of Traditional Chinese Medicine,may be spleen governing transportation and transformation of water,"speen-qi on dispersing jing and the essence belongs to the lung" and one of the embodiment and the molecular basis of normal distribution function of liquid water.It suggests that the lung and spleen have some correlation in the metabolism of water.关键词
肺为水之上源/湿阻中焦证/水通道蛋白Key words
Lung as the source of water/Syndrome of dampness in middle Jiao/Aquaporins分类
医药卫生引用本文复制引用
张凯文,黄秀深,陈芳,于海艳,王吉娥,张丰华..肺为水之上源理论与湿阻中焦证致肺水通道蛋白变化的关联性研究[J].四川中医,2018,36(2):56-60,5.基金项目
国家自然科学基金资助项目(编号:81373544). （编号:81373544）
