山东农业科学2018，Vol.50Issue(3)：1-5,5.DOI:10.14083/j.issn.1001-4942.2018.03.001
黑麦热激蛋白ScHsp90-1基因的克隆及表达分析
Cloning and Expression Analysis of Heat Shock Protein Gene ScHsp90-1 in Rye
摘要
Abstract
In this study, a Hsp90 gene was isolated from rye, named ScHsp90 -1.The full cDNA se-quence of ScHsp90-1 is 2 103 bp,which encodes 700 amino acids with a predicted molecular mass of 80.47 kD.The sequence analysis showed that ScHsp90-1 had higher homology with that of wheat,maize and rice. The phylogenetic analysis indicated that ScHsp90-1 was especially similar to TaHsp90 from wheat.The real-time quantitative PCR analysis showed that ScHsp90 -1 had response to high -temperature, cold, high -salt and drought treatments in rye.These results indicated that ScHsp90-1 gene might be a stress-related gene of rye.关键词
黑麦/热激蛋白Hsp90/克隆/表达分析Key words
Rye/Heat-shock protein Hsp90/Cloning/Expression analysis分类
农业科技引用本文复制引用
王利彬,张玉梅,穆平,赵振东,曹新有,董爽爽,王灿国,程敦公,李豪圣,刘爱峰,宋健民,刘建军,刘成..黑麦热激蛋白ScHsp90-1基因的克隆及表达分析[J].山东农业科学,2018,50(3):1-5,5.基金项目
山东省自然科学基金项目(ZR2016CQ33) （ZR2016CQ33）
国家重点研发计划项目(2016YFD0101802,2016YFE0108600) （2016YFD0101802,2016YFE0108600）
山东省农业科学院青年英才计划项目 （）
山东省农业科学院农业科技创新工程项目(CXGC2016A11,CXGC2016B01) （CXGC2016A11,CXGC2016B01）
旱区作物逆境生物学国家重点实验室开放课题(CSBAA2016008) （CSBAA2016008）
