山东农业科学2018，Vol.50Issue(3)：6-11,6.DOI:10.14083/j.issn.1001-4942.2018.03.002
甜樱桃MYB转录因子基因的克隆与表达分析
Cloning and Expression Analysis of MYB Transcription Factor Gene in Sweet Cherry
摘要
Abstract
Based on our previous transcriptome data of sweet cherry fruit, the MYB transcription factor gene was cloned using RT-PCR method from sweet cherry cultivar'Tieton',and named PaMYB10.The se-quence was submitted to GenBank with the accession number of ALH21137.1.Sequence analysis revealed that the deduced amino acid of PaMYB10 contained R2 and R3 conserved domain and it was a typical R2R3-MYB family gene.The encoded protein PaMYB10 had the closest genetic relationship with Prunus persica, Prunus domestica and Prunus mume.The result of qRT-PCR showed that PaMYB10 was expressed in stem, young leaf,flower and fruit tissues in red cultivar'Tieton',but the expression quantity was different.The ex-pression quantity of PaMYB10 was the highest in the mature fruit of 'Tieton'.The content of anthocyanin in'Tieton' increased gradually and the expression level of PaMYB10 showed significantly up-regulated along with the fruit development.However, the content of anthocyanin and expression level of PaMYB10 in the yellow cultivar'13-33' were almost unchanged and remained at a very low level during fruit ripening.In the later stage of fruit development,the content of anthocyanin and expression level of PaMYB10 in the red culti-var'Tieton' were significantly higher than those of the yellow cultivar '13-33'.It is speculated that the high level expression of PaMYB10 may associated with the anthocyanin accumulation in red sweet cherry fruit.关键词
甜樱桃/花青苷/转录因子/MYB/基因表达Key words
Sweet cherry/Anthocyanin/Transcription factor/MYB/Gene expression分类
农业科技引用本文复制引用
魏海蓉,宗晓娟,朱东姿,谭钺,徐丽,陈新,王甲威,刘庆忠..甜樱桃MYB转录因子基因的克隆与表达分析[J].山东农业科学,2018,50(3):6-11,6.基金项目
山东省现代农业产业技术体系果品产业创新团队专项基金项目(SDAIT-06-04) （SDAIT-06-04）
泰安市农业良种工程项目(2016LZ009) （2016LZ009）
山东省农业科学院青年科研基金项目(2014QNM33) （2014QNM33）
