山东农业科学2018，Vol.50Issue(3)：22-28,7.DOI:10.14083/j.issn.1001-4942.2018.03.005
基于SRAP分子标记的23株大球盖菇遗传多样性和亲缘关系分析
Genetic Diversity and Phylogenetic Relationship Analysis of 23 Stropharia rugosoannulata Strains Based on SRAP Molecular Marker
摘要
Abstract
Sequence-related amplified polymorphism(SRAP)molecular marker technique was used to study the genetic diversity and phylogenetic relationship among 23 Stropharia rugosoannulata strains collected from home and abroad.A total of 288 pairs of SRAP primers were tested,and 38 pairs could produce high-quality PCR production with good stability,repeatability and polymorphism.The analysis of SRAP amplifica-tion pattern showed that 322 bands were amplified from genomic DNA of 23 strains with the size of 100~2 000 bp,of which 205 bands were polymorphic and the percentage of polymorphic sites was 63.66%.The genetic similarity coefficient among the tested strains was ranged from 0.6646~0.9658.The cluster analysis procee-ded by UPGMA method showed that 23 strains could be divided into three major groups at the similarity coeffi-cient of 0.84.GroupⅠincluded two strains from abroad;GroupⅡincluded three high temperature resistant strains domesticated by our laboratory;Group Ⅲwere 18 strains cultivated or preserved in different cities in China.The results showed that significant genetic diversity existed among the strains.SRAP molecular marker technique could identify 23 strains clearly and analyze the phylogenetic relationship among different strains.This test provides the theoretical basis for the identification of Stropharia rugosoannulata phylogenetic relation-ship and new strain breeding.关键词
大球盖菇/SRAP分子标记/遗传多样性/亲缘关系Key words
Stropharia rugosoannulata/SRAP molecular marker/Genetic diversity/Phylogenetic rela-tionship分类
农业科技引用本文复制引用
朱静娴,李丽君,赵淑芳,陈宸,姜淑霞..基于SRAP分子标记的23株大球盖菇遗传多样性和亲缘关系分析[J].山东农业科学,2018,50(3):22-28,7.基金项目
山东省农业良种工程项目(2016LZGC026) （2016LZGC026）
山东省星火科技示范项目(2015XH019) （2015XH019）
