山东农业科学2018，Vol.50Issue(3)：29-32,4.DOI:10.14083/j.issn.1001-4942.2018.03.006
转EPSPS基因抗除草剂棉花在杂交制种中的应用初探
Application of EPSPS Transgenic Cotton with Herbicide Resistance in Hybrid Seed Production
摘要
Abstract
In this paper,a simplified method of cotton hybrid seed production was discussed.The EP-SPS transgenic cotton with herbicide resistance was used as the male parent, and the normal upland cotton germplasm was used as the female parent to carry out cotton seed production through direct pollination without castration,and then the F1hybrids with herbicide resistance were screened out at seedling stage.The SSR mo-lecular marker detection results showed that the simplified seed production method could produce 100%pure hybrids.This method can be further improved and popularize in the hybrid seed production of cotton with the combination of seedling nursing with nutrient bowl, which will have important utilization value in simplified seed production technology.关键词
棉花/EPSPS基因/除草剂/赤霉素/简化制种Key words
Cotton/EPSPS gene/Herbicide/Gibberellin/Simplified seed production分类
农业科技引用本文复制引用
高明伟,王秀丽,张超,陈莹,姜辉,王家宝,柴启超,王永翠,赵军胜..转EPSPS基因抗除草剂棉花在杂交制种中的应用初探[J].山东农业科学,2018,50(3):29-32,4.基金项目
国家重点研发计划项目(2016YFD0101400) （2016YFD0101400）
山东省重点研发计划项目(2016GNC110011) （2016GNC110011）
山东省农业科学院农业科技创新工程项目(CXGC2017B01) （CXGC2017B01）
