山东农业科学2018，Vol.50Issue(3)：107-112,6.DOI:10.14083/j.issn.1001-4942.2018.03.024
烟草根际溶磷解钾细菌的筛选鉴定及对烟草的促生作用
Screening and Identification of Phosphate-and Potassium-Solubilizing Bacteria and Their Growth Promoting Effect on Tobacco Seedlings
摘要
Abstract
In this experiment, the soil of Weifang tobacco field in Shandong was taken as the research object,the phosphate-and potassium-solubilizing bacteria in the rhizosphere were screened and identified and their effects on the growth of tobacco were analyzed.The results showed that 34 bacteria isolates were ob-tained from tobacco rhizosphere soil and 13 strains showed better antagonistic effects to both Phytophthora para-sitica and Ralstonia solanacearum.The phosphate-and potassium-solubilizing capabilities of those isolates were tested,and 8 strains showed better phosphate-solubilizing activity and 5 strains showed potassium-solu-bilizing activity.The highest content of soluble phosphate and available potassium was 241 mg/L and 13.95 mg/L respectively.Five strains showed the activity to produce indole acetic acid(IAA)and the highest con-centration of IAA was 104.71 μg/mL.H1-2 and 3183 strains could obviously promote the growth of tobac-co.The aboveground and underground fresh weight of tobacco seedlings irrigating with these bacteria solution were both higher than those of control.Through comparison of the 16S ribosomal DNA and phylogenetic analy-sis,3183 and 1441 strains belonged to Bacillus velezensis,H1-2 strain belonged to Peanibacillus polymyxa, and G2-7 and D2 strains belonged to Burkholderia spp.respectively.关键词
烟草/植物根际促生菌/溶磷/解钾/鉴定Key words
Tobacco/Plant growth-promoting rhizobacteria/Phosphate solubilization/Potassium solubili-zation/Identification分类
农业科技引用本文复制引用
徐立国,方换男,肖云峰,王汝法,刘爱新,李现道,刘春菊..烟草根际溶磷解钾细菌的筛选鉴定及对烟草的促生作用[J].山东农业科学,2018,50(3):107-112,6.基金项目
山东省烟草公司面上项目 （）
