山东医药2018，Vol.57Issue(7)：1-4,4.DOI:10.3969/j.issn.1002-266X.2018.07.001
miR-381在肾癌细胞中的表达变化及其对细胞增殖、迁移、侵袭能力的影响
Expression of miR-381 and its effect on proliferation,migration, and invasion of renal carcinoma cells
摘要
Abstract
Objective To observe the expression changes of miR-381 in the renal cell carcinoma cell line, and its effect on the proliferation,migration,and invasion of renal cell carcinoma cells.Methods The expression of miR-381 in three renal cell carcinoma cell lines(Caki-2,A498,and 786-O)and normal kidney cell line HK-2 was detected by fluo-rescent quantitative PCR.The Caki-2 cell line was divided into three groups,miR-381 group which was transfect with miR-381 mimics,mock group which ws transfected with miR-381,and the NC group without transfection.CCK-8,Scratch test, and Transwell assay were used to determine the proliferation,migration,and invasion capabilities.The real-time PCR and Western blotting were used to detect the mRNA and protein levels of ID-1 in these three groups.Results The expression level of miR-381 in the Caki-2,A498 and 786-O cells was significantly lower than that in the HK-2 cells(P<0.01).At 72 and 96 h after transfection,the proliferation of miR-381 group was lower than that of the NC group and mock group(P<0.01).The wound healing rate was 28.7 ±2.6%in the miR-381 group,56.4 ±4.5% in the NC group, and 59.7 ± 4.8%in the mock group, and the wound healing rate of the miR-381 group was significantly lower than that in the NC group and mock group(all P<0.01).The invasive cells were 44.7 ±3.9 in the miR-381 group,225.7 ±15.7 in the NC group,and 235.9 ±17.9 in the mock group,and the invasive cells were significantly less than those of the NC group and mock group(all P<0.01).Compared with the NC and mock groups, the mRNA and protein expression of ID-1 in the cells with over-expression of miR-381 was lower(P<0.01).Conclusions The miR-381 is low expressed in the renal cell carcinoma cells.Over-expression of miR-381 can inhibit the proliferation,migration,and invasion of renal cell carci-noma cells,which may be associated with the down-regulated expression of ID-1.关键词
肾癌/微小RNA381/DNA结合抑制因子1/细胞增殖/细胞迁移/细胞侵袭Key words
renal cell carcinoma/microRNA-381/ID-1/cell proliferation/cell migration/cell invasion分类
医药卫生引用本文复制引用
梁颖莹,莫志文,黄宇帆,邵汛帆,袁亚维..miR-381在肾癌细胞中的表达变化及其对细胞增殖、迁移、侵袭能力的影响[J].山东医药,2018,57(7):1-4,4.基金项目
国家自然科学基金青年项目(81502194) （81502194）
广东省科技公益专项(2014A020212513) （2014A020212513）
广州医科大学博士启动项目(2014C49). （2014C49）
