山东医药2018，Vol.57Issue(7)：9-13,5.DOI:10.3969/j.issn.1002-266X.2018.07.003
三阴型、Luminal型、HER2阳性型乳腺癌瘤组织中FA/BRCA通路基因表达对比观察
Comparison in expression of FA/BRCA pathway genes in triple-negative,Luminal, and HER2 over-expression subtypes of breast cancer tissues
摘要
Abstract
Objective To observe the differential expression of Fanconi anemia FA /BRCA pathway genes in different subtypes of breast cancer tissues and to explore the significance.Methods Ninety-three breast cancer tissues from inva-sive ductal carcinoma were collected during surgical resection.Among them, there were 20 cases of triple-negative breast cancer tissues,20 cases of Luminal breast cancer tissues,and 20 cases of HER2-positive breast cancer tissues.The num-ber of three types of breast cancer after neoadjuvant chemotherapy were 11,13 and 9 cases, respectively.The real-time PCR was used to detect the mRNA expression of genes(FANCA, FANCD2, FANCF, FANCI, BRCA1, and BRCA2) from the FA/BRCA pathway in the breast cancer tissues.Results The expression of BRCA1, BRCA2, FANCD2, and FANCI was significant higher in HER2-positive type breast cancer tissues than that of the other two subtype cancer tissues;the expression level of FANCA from Luminal breast cancer tissues was higher than those in the HER 2-positive type and tri-ple-negative type breast cancer tissues(all P<0.05).The expression of five genes from the FA/BRCA pathway except FANCF was low in neoadjuvant chemotherapeutic triple-negative breast cancer tissues.After neoadjuvant chemotherapy,the expression levels of BRCA1,BRCA2,FANCA,and FANCI from Luminal type breast cancer tissues were higher than those in the HER2-positive type and triple-negative type breast cancer tissues(all P<0.05).The expression levels of BRCA1, BRCA2,FANCD2,and FANCI in the triple-negative type and HER2-positive type patients who underwent neochemothera-py were lower than those in the patients who underwent the surgery directly,while the expression of FANCF remained high after neochemotherapy(all P<0.05).Conclusions Distinct expression pattern of FA-related genes in three types of breast cancer was found in our study.The role of FA/BRCA pathway gene in HER2-type breast cancer tissues is different from that in the other two subtypes.The high level expressions of BRCA 1,BRCA2,FANCA and FANCI may relate to the response of Luminal subtype to neoadjuvant chemotherapy.关键词
乳腺肿瘤/三阴型乳腺癌/范科尼贫血/乳腺肿瘤易感基因Key words
breast neoplasms/triple negative breast neoplasms/Fanconi anemia/BRCA1 protein/BRCA2 protein分类
医药卫生引用本文复制引用
马芸,段文晶,李姝墨,董坚,杨莹..三阴型、Luminal型、HER2阳性型乳腺癌瘤组织中FA/BRCA通路基因表达对比观察[J].山东医药,2018,57(7):9-13,5.基金项目
云南省卫生系统领军人才培养计划项目(L-201212). （L-201212）
